Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1992-09-14
1999-03-23
LeGuyader, John
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351723, 43525233, 4353201, 536 231, 536 237, C12N 1531, C12N 1570, C12N 1511
Patent
active
058857923
ABSTRACT:
A DNA sequence of high biotin operon-expression system usable for breeding a bacterium having excellent biotin productivity is provided.
A DNA sequence of biotin operon characterized in the fact that at least one base pair of either a nucleotide sequence of the regulatory region of the biotin operon of Escherichia coli or a nucleotide sequence in the vicinity of the bioB initiating codon is mutated in comparison with that of the one in its wild type strain is provided. Escherichia coli transformed with a recombinant plasmid carrying such a DNA sequence has high biotin-productivity.
REFERENCES:
G. Ketner et al., "Operator and Promotoer Mutations Affecting Divergent Transcription in the bio Gene Cluster of Escherichia coli", J. Mol. Biol. (1975) vol. 96, pp. 13-27.
Chemical Abstracts, vol. 108, No. 15, Apr. 11, 1988, Abstract No. 125889, p. 199.
Otsuka et al., "The Regulatory Region of the Biotin Operon in Escherichia coli", Nature, vol. 276 Dec. 14, 1978, pp. 689-694.
Otsuka et al., "The Escherichia coli Biotin Biosynthetic Enzyme Sequences Predicted from the Nucleotide Sequence of the . . . ", J. Biol. Chem., vol. 263, No. 36, Dec. 25, 1988, pp. 19577-1958.
Barker et al., "Sequence and Properties of Operator Mutations in the Bio Operon of Escherichia coli", Gene, vol. 13, pp. 89-102, 1981.
Haze Shinitiro
Ifuku Ohji
Kishimoto Jiro
Nakahama Kazuo
LeGuyader John
Shiseido Company Ltd.
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