Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2009-01-30
2011-10-25
Nashed, Nashaat (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S069700
Reexamination Certificate
active
08043830
ABSTRACT:
The present invention provides methods of metabolically biotinylating recombinant proteins. Cell lines and specific protein and nucleic acid constructs for use in the methods of the present invention are also provided herein.
REFERENCES:
patent: 6903203 (2005-06-01), Copley et al.
patent: 2002/0142355 (2002-10-01), Barry et al.
patent: WO 00/05406 (2000-02-01), None
Yazaki et al. Mammalian expression and hollow fiber bioreactor production of recombinant anti-CEA diabody and minibody for clinical application. J. Immunological Method (2001) 253, 195-208.
Barat, B. et al., “Metabolic biotinylation of recombinant antibody by biotin ligase retained in the endoplasmic reticulum,”Biomolecular Engineering,2007, vol. 24, pp. 283-291.
Nesbeth, D. et al., “Metabolic Biotinylation of Lentiviral Pseudotypes for Scalable Paramagnetic Microparticle-Dependent Manipulation,”Molecular Therapy,Apr. 2006, vol. 13, No. 4, pp. 814-822.
Parrott, M.B. et al., “Metabolic Biotinylation of Recombinant Proteins in Mammalian Cells and in Mice,”Molecular Therapy,Jan. 2000, vol. 1, No. 1, pp. 96-104.
Parrott, M.B. et al., “Metabolic Biotinylation of Secreted and Cell Surface Proteins from Mammalian Cells,”Biochemical and Biophysical Research Communications,2001, vol. 281, No. 4, pp. 993-1000.
Verhaegen, M. et al., “RecombinantGaussiaLuciferase. Overexpression, Purification, and Analytical Application of a Bioluminescent Reporter for DNA Hybridization,”Analytical Chemistry,Sep. 1, 2002, vol. 74, No. 17, pp. 4378-4385.
Barat Bhaswati
Wu Anna M.
Morgan & Lewis & Bockius, LLP
Nashed Nashaat
Parent Annette S.
The Regents of the University of California
LandOfFree
Biotin-ligase system for secretion of biotinylated protein does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Biotin-ligase system for secretion of biotinylated protein, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Biotin-ligase system for secretion of biotinylated protein will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4278662