Biotin-binding containment systems

Details Biotin-binding containment systems Biotin-binding containment systems

1995-05-01

1997-10-28

Ketter, James

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

435410, 43525231, 43525233, 4352572, 4353201, 536 237, 536 241, C12N 510, C12N 113, C12N 121

Patent

active

056817452

ABSTRACT:
The present invention relates to genetic containment systems which express a biotin-binding component that can be used for selectively destroying recombinant cells such as genetically engineered microorganisms. These systems may comprise a streptavidin or an avidin gene whose expression is controlled by a regulatable promoter. The regulatory agent such as a transcriptional effector is expressed from another gene which may also be expressed and its expression controlled by the containment system. Expression of the agent can be designed to respond to physiological changes in the environment. The invention also relates to containment systems and methods for the selective detection or tracking of recombinant cells and to eukaryotic and prokaryotic cells which contain these genetic containment systems.

REFERENCES:
McClure et al., "The Construction of Broad Host Range Genetic Markers and eir use in Monitoring the Release of Catabolic Gems to Aquatic Environments", Proc.--Eur. Congr. Biotechnol, 5th (1990), vol. 1, pp. 169-172.
Molin et al., "Suicidal genetic elements and their use in biological containment of bacteria", Annu. Rev. Microbiol. 47: 139-166, 1993.
Sano et al., "A streptavidin-metallothionen chimera that allows specific labeling of biological materials with many different heavy metal ions", Proc. Nat. Acad. Sci. USA, 89: 1534-1538, Mar. 1992.
Sano and Cantor, "Expression of a cloned streptavidin gene in Escherichia coli", Proc. Nat. Acad. Sci. USA, 87: 142-146, Jan. 1990.

Affiliated with

Also associated with

Rating

Biotin-binding containment systems does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Biotin-binding containment systems, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Biotin-binding containment systems will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1025647