Biotin-binding containment systems

Details Biotin-binding containment systems Biotin-binding containment systems

1995-06-07

1997-10-21

Ketter, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435732, 435 737, 4351723, 435262, 4352625, 4353201, G01N 3353

Patent

active

056795335

ABSTRACT:
The present invention relates to genetic containment systems which express a biotin-binding component that can be used for selectively destroying recombinant cells such as genetically engineered microorganisms. These systems may comprise a streptavidin or an avidin gene whose expression is controlled by a regulatable promoter. The regulatory agent such as a transcriptional effector is expressed from another gene which may also be expressed and its expression controlled by the containment system. Expression of the agent can be designed to respond to physiological changes in the environment. The invention also relates to containment systems and methods for the selective detection or tracking of recombinant cells and to eukaryotic and prokaryotic cells which contain these genetic containment systems.

REFERENCES:
Sano et al., "Expression of a cloned streptavidin gene in Escherichia col Proc. Nat. Acad. Sci. USA, 87: 142-146 Jan. 1990.
Molin et al., "Suicidal genetic elements and their use in biological containment", Annu. Rev. Microbiol. 47: 139-166 1993.

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