Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1993-11-15
1996-01-30
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 72, 435 721, 435 79, 435960, 435962, 435967, 435975, G01N 33535
Patent
active
054879752
ABSTRACT:
The present invention provides an improved biotin-avidin formulation in which a biotinylated antibody conjugate or an avidin-enzyme conjugate is present in a suitable diluent for immunohistochemical staining. The diluent additionally comprises casein in an amount sufficient to prevent charge interactions of the conjugate with a tissue section and gamma globulin in an amount sufficient to prevent Fc receptor binding and any hydrophobic interaction of the conjugate with a tissue section. In a preferred embodiment, the immunoglobulin is from the same species as the biotinylated antibody conjugate. The formulation effectively reduces overall unwanted binding, irrespective of the source of the binding.
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Druyor Roberta L.
Martel Ralph R.
Miller Phillip C.
Saunders David
Ventana Medical Systems, Inc.
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