Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-06-06
1998-12-01
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435183, 435 697, 4352523, 43525233, 4352525, 4352534, 4352535, 4353201, 435882, 530326, 530825, 536 232, 536 234, 536 237, C12P 2106, C12N 900, C12N 100, A61K 3800
Patent
active
058437096
ABSTRACT:
A bacterial host is described which is transformed by a plasmid coding for a polypeptide precursor wherein the host comprises a multi-enzyme complex capable of reacting with the expressed polypeptide precursor to produce a polypeptide comprising at least one dehydroamino acid and/or at least one lanthione bridge. A process for producing a polypeptide comprising at least one dehydroamino acid and/or at least one lanthione bridge, such as gallidermin, is also described. A plasmid capable of transforming a bacterial host is additionally described.
Also disclosed are recombinant DNA molecules which specify Epi B, Epi C, Epi D, Epi P and Epi Q, enzymes which are involved in the biosynthesis of lantibiotic epidermin.
REFERENCES:
patent: 4716115 (1987-12-01), Gonzalez et al.
patent: 5231013 (1993-07-01), Jung et al.
Allgaier et al., "Epidermin: Sequencing of a Heterodetic Tetracyclic 21-Peptide Amide Antibiotic", Eur. J. Biochem. 160(1):9-22 (1986).
Augustin et al., "Genetic Analysis of Epidermin Biosynthetic Genes and Epidermin-Negative Mutants of Staphylococcus epidermidis", Eur. J. Biochem. 204(3):1149-1154 (Mar., 1992).
Augustin et al., "Identification of Epidermin Biosynthetic Genes by Complementation Studies and Heterologous Expression", in: Proceedings of the 1st International Workshop on Lantibiotics. Nisin Novel Lantibiotics 1991, pp. 277-286 (1991).
Banerjee et al., "Structure and Expression of a gene Encoding the Precursor of Subtilin, a Small Protein Antibiotic", J. Biol. Chem. 263(19):9508-9514 (Jul. 5, 1988).
Buchman et al., "Structure, Expression, and Evolution of a Gene Encoding the Precursor of Nisin, a Small Protein Antibiotic", J. Biol. Chem. 263(31):16260-16266 (Nov. 5, 1988).
Entian et al., "Structure and dNA-Sequence Analysis of the Staphylococcal Lantibiotics Epidermin and Gallidermin", Abstr. of the Annual Meeting of Am. Soc. for Microbiol. 89(0):182, Abstr. No. H-78 (May, 1989).
Fiedler et al., "Purification of the Hydrophilic Antibiotics Epidermin, Gallidermin, and Nikkomycin Z by Preparative Reversed-Phase HPLC", Chem. Abstr. 110:583, Abstr. No. 210845v (Jan. 2, 1989).
Fiedler et al., "Purification of the Hydrophilic Antibiotics Epidermin, Gallidermin, and Nikkomycin Z by Preparative Reversed-Phase HPLC", Chromatographia 26:215-220 (Dec., 1988).
Gennaro et al., "A Site-Specific Recombination Function in Staphylococcus aureus Plasmids", J. Bacteriol. 169(6):2601-2610 (Jun., 1987).
Horinouchi et al., "Nucleotide sequence and Functional Map of pC194, a Plasmid that Specifies Inducible Chloramphenicol Resistance", J. Bacteriol. 150(2):815-825 (1982).
Jung, G., "Lantibiotics -- Ribosomally Synthesized Biologically Active Polypeptides Containing Sulfide Bridges and .alpha.,.beta.-Didehydroamino Acids", Angew. Chem. Int. Ed. Engl. 30 (9)1051-1068 (Sep., 1991).
Kaletta et al., "Pep5, a New Lantibiotic: Structural Gene Isolation and Prepeptide Sequence", Arch. Microbiol. 152(1):16-19 (Jun., 1989).
Kaletta, C. and Entian, K.-D., "Nisin, a Peptide Antibiotic: Cloning and Sequencing of the nisA Gene and Posttranslational Processing of its Peptide Product", J. Bacteriol. 171(3):1597-1601 (Mar., 1989).
Kaletta, C. et al., "Cloning of Genes Coding for Polypeptide Antibiotics (Lantibiotics) and Posttranslational Processing of Their Peptide Products", DECHEMA Biotechnology Conf. 3, Part A:33-35 (May, 1989).
Kellner et al., "Gallidermin: A New Lanthionine-Containing Polypeptide Antibiotic", Eur. J. Biochem. 177(1):53-59 (Feb. 1988).
Kellner et al., "Gallidermin: A New Lanthionine-Containing Polypeptide Antibiotic", Chem. Abstr. 110:395, Abstract No. 4243u (Jan. 2, 1989).
Khan et al., "Complete Nucleotide Sequence of pT181, a Tetracycline-Resistance Plasmid from Staphylococcus aureus", Plasmid 10:251-259 (1983).
Klein et al., "Analysis of Genes Involved in Biosynthesis of the Lantibiotic Subtilin", Appl. Env. Microbiol. 58(1):132-142 (1992).
Koide et al., "Cloning and Sequencing of the Major Intracellular Serine Protease Gene of Bacillus subtilis", J. Bacteriol. 167(1):110-116 (1986).
Kupke, T. et al., "Purification and Characterization of EpiD, a Flavoprotein Involved in the Biosynthesis of the Lantibiotic Epidermin", J. Bacteriol. 174(16):5354-5361 (Aug., 1992).
Schnell et al., "Prepeptide Sequence of Epidermin, A Ribosomally Synthesized Antibiotic with Four Sulfide-Rings", Chem. Abstr. 109:288, Abstract No. 144800c (Oct. 24, 1988).
Schnell et al., "Structural Gene Isolation and Prepeptide Sequence of Gallidermin, a New Lanthionine Containing antibiotic", FEMS Microbiol. Lett. 58(2/3):263-268 (Apr., 1989).
Schnell et al., "Prepeptide Sequence of Epidermin, a Ribosomally Synthesized Antibiotic with Four Sulphide-Rings", Nature 333:276-278 (May 19, 1988).
Schnell et al., "The Operon-Like Organisation of Lantibiotic Epidermin Biosynthesis Genes", in: Proceedings of the 1st International Workshop on Lantibiotics. Nisin Novel Lantibiotics 1991, pp. 269-276 (1991).
Schnell et al., "Analysis of Genes Involved in the Biosynthesis of the Lantibiotic Epidermin", Eur. J. Biochem. 204:57-68 (1992).
Shiba et al., "Lanthionine Peptide", Chem. Abstr. 104:206, Abstract No. 2246z (1986).
Shiba et al., "Lanthionine Peptide" (with English translation), Kagaku 40416-417 (1985).
Vieira et al., "The pUC Plasmids, an M13mp7-Derived System for Insertion Mutagenesis and Sequencing with Synthetic Universal Primers", Gene 19:259-268 (1982).
Yanisch-Perron et al., "Improved M13 Phage Cloning Vectors and Host Strains: Nucleotide Sequences of the M13mp18 and pUC19 Vectors", Gene 33:103-119 (1985).
European Search Report for European Patent Application No. EP 89 108907, corresponding to U.S. Application Serial No. 07/353,590, filed May 18, 1989, which is the great-great-grandparent of this application.
Shiba et al. (1986) Biopolymers 25, S11-S19.
Fiedler et al. (1988) Chromatographia 26, 215-220.
Augustin Johannes
Engelke Germar
Entian Karl-Dieter
Gotz Friedrich
Jung Gunther
Dr. Karl Thomae GmbH
Slobodyansky Elizabeth
Wax Robert A.
LandOfFree
Biosynthetic process for the preparation of chemical compounds does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Biosynthetic process for the preparation of chemical compounds, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Biosynthetic process for the preparation of chemical compounds will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2394927