Biosynthetic gene murD from streptococcus pneumoniae

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435183, 435243, 4352523, 4353201, 435325, 536 232, 935 22, C12N 1500, C12N 900, C12N 500, C07H 2104

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058342702

ABSTRACT:
The invention provides isolated nucleic acid compounds encoding the murD stem peptide biosynthetic gene of Streptococcus pneumoniae. Also provided are vectors and transformed heterologous host cells for expressing the MurD enzyme product and a method for identifying compounds that inhibit stem peptide biosynthesis.

REFERENCES:
patent: 5681694 (1997-10-01), Hoskins et al.
Mengin-Lecreulx, et al., "Organization of the murE-murG Region Of E. Coli", J. Bacteriol, 171:11, pp. 6126-6134 (1989).
M. Ikeda, et al., "Homology Among murC, murD, murE and murF Proteins in E. Coli and Thet Between E. Coli murG And A Possible murG Protein in B. Subtilis", J. Gen. Appl. Microbiol., 36, pp. 179-187 (1990).
Daniel, et al., "DNA sequence of the murE-murD region of Bacillus subtilis 168", J. Gen. Microbiol., 139 (2); 361-370, (1993).
Leach et al., "Rhizobium meliloti homologs of Escherichia coli mur genes", Gene, 148, pp. 87-90, (1994).
Fleischmann et al., "Whole-genome random sequencing and assembly of Haemophilus Influenzae Rd", Science, 269, pp. 496-498 and 507-512, (1995).

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