Biosynthetic antibody binding sites

Details Biosynthetic antibody binding sites Biosynthetic antibody binding sites

1991-01-02

1992-02-25

Doll, John

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Blood proteins or globulins, e.g., proteoglycans, platelet...

530388, 536 27, 435 696, 435 697, 435 7021, 4351722, 4351723, 43524027, 4353201, C07K 1528, C07H 2104

Patent

active

050915134

ABSTRACT:
Disclosed are a family of synthetic proteins having affinity for a preselected antigen. The proteins are characterized by one or more sequences of amino acids constituting a region which behaves as a biosynthetic antibody binding site (BABS). The sites comprise 1) non-covalently associated or disulfide bonded synthetic V.sub.H and V.sub.L dimers, 2) V.sub.H -V.sub.L or V.sub.L -V.sub.H single chains wherein the V.sub.H and V.sub.L are attached by a polypeptide linker, or 3) individuals V.sub.H or V.sub.L domains. The binding domains comprise linked CDR and FR regions, which may be derived from separate immunoglobulins. The proteins may also include other polypeptide sequences which function e.g., as an enzyme, toxin, binding site, or site of attachment to an immobilization media or radioactive atom. Methods are disclosed for producing the proteins, for designing BABS having any specificity that can be elicited by in vivo generation of antibody, and for producing analogs thereof.

REFERENCES:
patent: 4355023 (1982-10-01), Ehrlich
patent: 4474893 (1984-10-01), Reading
patent: 4642334 (1987-02-01), Moore
patent: 4666837 (1987-05-01), Harford et al.
patent: 4704692 (1987-11-01), Ladner
patent: 4816397 (1989-03-01), Boss
patent: 4816567 (1989-03-01), Cabilly
patent: 4946778 (1990-08-01), Ladner et al.
Arnon (1986) Chemical Abstracts 105(5), p. 1, Ab. No. 34903y.
Baer et al. (1985) Cell, 43:705-713.
Boulianne et al. (1984) Nature, 312:543-646.
Boulianne et al. (1987) Mol. Biol. Med., 4:37-49.
Brown et al. (1987) Cancer Res., 47:3577-3583.
Cabilly et al. (1984) Proc. Natl. Acad. Sci. U.S.A., 81:3273-3277.
Corvalen and Smith (1987) Cancer Immunol. Immunother., 24:127-132.
Corvalen et al. (1987) Cancer Immunol. Immunother., 24:133-137.
Dammacco et al. (1972) J. Immunol., 109:565-569.
Erlich et al. (1980) Biochem., 19:4091-4096.
Gascoigne et al. (1987) Proc. Natl. Acad. Sci. U.S.A., 84:2936-2940.
Greenberg (1986) G. E. News, Dec.
Haber (1983) Biochem. Pharmacol., 32:1967-1977.
Haber and Novotny (1985) "The Antibody Combining Site" in Hybridoma Technology in the Biosciences and Medicine, Plenum Publishing Corp., pp. 57-76.
Hochman et al., (1973) Biochem., 12:1130-1135.
Huston et al. (1972) Biochem., 11:4256-4262.
Inbar et al. (1972) Proc. Natl. Acad. Sci. U.S.A., 69:2659-2662.
Itakura et al. (1977) Science, 198:1056-1063.
Jones et al. (1986) Nature, 321:522-525.
Kabat et al. (1978) Proc. Natl. Acad. Sci. U.S.A., 75:2429-2433.
Kabat et al. (1979) Sequence of Immunoglobulin Chains, NIH Publication No. 80-2008, pp. 1-107.
Klausner (1986) Biotechnology, 4:1041-1043.
Liu et al. (1987) Gene, 54:33-40.
Liu et al. (1987) Proc. Natl. Acad. Sci. U.S.A., 84:3439-3443.
Marx (1985) Science, 299: 455-456.
Morrison (1985) Science, 299:1202-1207.
Morrison et al. (1984) Proc. Natl. Acad. Sci. U.S.A., 81:6851-6855.
Neuberger et al. (1984) Nature, 312:604-608.
Neuberger (1986) Chemical Abstracts 105(11), p. 475, Ab. No. 95609d.
Nishimuru et al. (1987) Cancer Res., 47:999-1005.
Ohno et al. (1985) Proc. Natl. Acad. Sci. U.S.A., 82:2945-2949.
Pollack et al. (1986) Science, 234:1570-1573.
Rice and Baltimore (1982) Proc. Natl. Acad. Sci. U.S.A., 79:7862-7865.
Richardson (1987) Chemical Abstracts 107(5), p. 330, Ab. No. 35697n.
Rosemblatt and Haber (1978) Biochem., 17:3877-3882.
Sahagan et al., (1986) J. Immunol., 137:1066-1074.
Schultz (1988) Science, 240:426-432.
Sharon and Givol (1976) Biochem., 15:1591-1594.
Sun et al. (1987) Proc. Natl. Acad. Sci. U.S.A., 84:214-218.
Takeda et al. (1985) Nature, 314:452-454.
Tan et al. (1985) J. Immunol., 135:3564-3567.
Thiesen (1987) Chemical Abstracts 106(13), p. 37, Ab. No. 95759y.
Tonegawa et al. (1977) Proc. Natl. Acad. Sci. USA, 74:3518-3522.
Tramontano et al. (1986) Science, 234:1566-1570.
Van Brunt (1986) Biotechnology, 4:277-283.
Vogel (1987) "Current Approaches of Immunotargeting in Immunoconjugates" in Antibody Conjugates in Radioimaging and Therapy of Cance
The United States Government has certain rights in this application as the subject matter hereof was developed in part using funds from SBIR Grant Nos. SSS-4 1 R43 CA39870-01 and SSS-4 2 R44 CA39870-02.

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