Biosynthetic antibody binding sites

Details Biosynthetic antibody binding sites Biosynthetic antibody binding sites

1993-10-19

1995-12-19

Budens, Robert D.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 696, 435 697, 4351723, 4352402, 4352523, 4353201, C12N 121, C12N 510, C12N 1509

Patent

active

054767865

ABSTRACT:
Disclosed are a family of synthetic proteins having affinity for a preselected antigen. The proteins are characterized by one or more sequences of amino acids constituting a region which behaves as a biosynthetic antibody binding site (BABS). The sites comprise 1) non-covalently associated or disulfide bonded synthetic V.sub.H and V.sub.L dimers, 2) V.sub.H -V.sub.L or V.sub.L -V.sub.H single chains wherein the V.sub.H and V.sub.L are attached by a polypeptide linker, or 3) individual V.sub.H or V.sub.L domains. The binding domains comprise linked CDR and FR regions, which may be derived from separate immunoglobulins. The proteins may also include other polypeptide sequences which function e.g., as an enzyme, toxin, binding site, or site of attachment to an immobilization media or radioactive atom. Methods are disclosed for producing the proteins, for designing BABS having any specificity that can be elicited by in vivo generation of antibody, and for producing analogs thereof.

REFERENCES:
patent: 4355023 (1982-10-01), Ehrlich et al.
patent: 4474893 (1984-10-01), Reading
patent: 4642334 (1987-02-01), Moore et al.
patent: 4666837 (1987-05-01), Harford et al.
patent: 4704692 (1987-11-01), Ladner
patent: 4946778 (1990-08-01), Ladner et al.
patent: 5091513 (1992-02-01), Huston et al.
patent: 5132405 (1992-07-01), Huston et al.
patent: 5225539 (1993-07-01), Winter
patent: 5258498 (1993-11-01), Huston et al.
patent: 5260203 (1993-11-01), Ladner et al.
Kabat et al. (1979) "Sequence of Immunoglobulin Chains," NIH Publication No. 80-2008 pp. 1-107.
Klausner, A. (1986) "`Single-Chain` Antibodies Become a Reality," Biotechnology 4:1041-1043.
Nishimura et al. (1987) "Recombinant Human-Mouse Chimeric Monoclonal Antibody Specific for Common Acute Lymphocytic Leukemia Antigen," Cancer Research 47:999-1005.
Tonegawa et al. (1977) "Cloning of an Immunoglobulin Variable Region Gene from Mouse Embryo," Proc. Natl. Acad. Sci. USA 74:3518-3522.
Verhoeyen et al. (1988) "Reshaping Human Antibodies: Grafting an Antilysozyme Activity," Science 239:1534-1536.
Huston et al. (1972) Biochemistry, vol. 11, No. 23, pp. 4256-4262.
Dammacco et al. (1972) The Journal of Immunology, vol. 109, No. 3, pp. 565-569.
Inbar, et al. (1972) Proc. Nat. Acad. Sci. vol. 69, No. 9, pp. 2659-2662.
Hochman, et al. (1973) Biochemistry, vol. 12, No. 6, pp. 1130-1135.
Sharon et al. (1976) Biochemistry, vol. 15, No. 7, pp. 1590-1594.
Itakura et al. (1977) Science, vol. 198, pp. 1056-1063.
Rosemblatt et al. (1978) Biochemistry, vol. 17, No. 18, pp. 3877-3882.
Kabat et al. (1978) Proc. Natl. Acad. Sci. vol. 75, No. 5, pp. 2429-2433.
Ehrlich et al. (1980) Biochemistry, vol. 19, No. 17, pp. 4091-4096.
Wetzel et al. (1981) Gene, vol. 16, pp. 63-71.
Rice et al. (1982) Proc. Natl. Acad. Sci, vol. 79, pp. 7862-7865.
Haber, Biochemical Pharmacology, vol. 32, No. 13, pp. 1967-1977.
Morrison et al., (1984) Proc. Natl. Acad. Sci., vol. 81, pp. 6851-6855.
Cabilly et al. (1984) Proc. Natl. Acad. Sci., vol. 81, pp. 3273-3277.
Neuberger et al. (1984) Nature, vol. 312, pp. 604-608.
Boulianne et al. (1984) Nature, vol. 312, pp. 643-646.
Haber et al. (1985) Chapter 3, The Antibody Combining Site, pp. 57-76.
Takeda et al. (1985) Nature, vol. 314, pp. 452-454.
Marx (1985), Science, vol. 229, pp. 455-456.
Tan et al. (1985) The Journal of Immunology, vol. 135, No. 5, pp. 3564-3567.
Ohno et al. (1985) Proc. Natl. Acad. Sci., vol. 82, pp. 2945-2949.
Morrison (1985) Science, vol. 229, pp. 1202-1207.
Baer et al. (1985) Cell, vol. 43, pp. 705-513.
Sun et al. (1986) Hybridoma, vol. 5, Suppl. 1, pp. S17-S20.
Van Brunt (1986) Biotechnology, vol. 4, pp. 277-283.
Arnon (1986) Chemical Abstracts, vol. 105, No. 5, p. 1.
Neuberger (1986) Chemical Abstracts, vol. 105, No. 11, p. 475.
Jones et al. (1986) Nature, vol. 321, pp. 522-525.
Pollack et al. (1986) Science, vol. 234, pp. 1570-1573.
Tramontano et al. (1986) Science, vol. 234, pp. 1566-1570.
Sahagan et al. (1986) The Journal of Immunology, vol. 137, No. 3, pp. 1066-1074.
Williams et al. (1986) Gene, vol. 43, pp. 319-324.
Genex makes a miniaturized antibody (1986) Newswatch, p. 5.
Sun, et al. (1987) Proc. Natl. Acad. Sci., vol. 84, pp. 214-218.
Greenberg, Genex Designs Single-Chain Antibodies, Patents Sought.
Liu et al. (1987) Proc. Natl. Acad. Sci., vol. 84, pp. 3439-3443.
Liu et al. (1987) Gene, vol. 54, pp. 34-40.
Thiesen (1987) Chemical Abstracts, vol. 106, p. 37, Ab. No. 95759y.
Richardson (1987) Chemical Abstracts, The American Chemical Society vol. 107, No. 5, p. 330, Ab. No. 35697n.
Chothia et al. (1987), J. Mol. Biol. vol. 196, pp. 901-917.
Corvalan et al. (1987) Cancer Immunol Immunother, vol. 24, pp. 127-132.
Vogel (1987) Current Approaches of Immunotargeting, New York, Oxford University Press, pp. 3-7.
Brown et al. (1987) Cancer Research, vol. 47, pp. 3577-3583.
Boulianne et al. (1987) Mol. Biol. Med. vol. 4, pp. 37-49.
Gascoigne et al. (1987) Proc. Natl. Acad. Sci., vol. 84, pp. 2936-2940.
Corvalan et al. (1987) Cancer Immunol Immunother, vol. 24, pp. 133-137.
Schultz (1987) Science, vol. 240, pp. 426-432.

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