Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Reexamination Certificate
2006-04-25
2006-04-25
Prouty, Rebecca E. (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
C530S387300, C435S328000, C435S254200, C435S325000, C435S419000, C435S189000, C536S023600, C536S023200
Reexamination Certificate
active
07033815
ABSTRACT:
The present invention makes use of a chimeric gene that, when incorporated into an appropriate host, results in the overproduction of S-adenosylmethionine without the need to supply the host with a source of untransformed methionine. The need for the methionine source is eliminated because the appropriately chosen host manufactures the amino acid on its own, and when the host is modified by the inclusion of a chimeric gene of the present invention, it transforms the methionine that is produced into S-adenosylmethionine. In addition, the same chimeric gene causes accumulation of methionine and increase in folate content in the same host. One form of the present invention is a fused gene encoding for methylene tetrahydrofolate reductase (MTHFR) made up of an N-terminal domain from a yeast organism and a C-terminal domain from a plant species. An example of a suitable plant species isArabidopsis thaliana. An example of a suitable yeast organism isSaccharomyces cerevisiae.
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Appling Dean R.
Hanson Andrew D.
Raymond Rhonda K.
Roje Sanja
Board of Regents the University of Texas System
Gardere Wynne & Sewell LLP
Prouty Rebecca E.
University of Florida
Walicka Malgorzata A.
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