Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1991-10-21
1999-01-05
LeGuyader, John L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 737, 435 772, 435 29, 435 34, 435 41, C12Q 100, C12Q 104, C12Q 126
Patent
active
058561080
ABSTRACT:
There is provided a method for producing diazoluminomelanin (DALM) which comprises culturing in a medium containing nitrate, 3-amino-L-tyrosine (3-AT) and luminol under suitable metabolic conditions, a microorganism containing nitrate reductase.
Also provided is a method for directly detecting microorganisms containing nitrate reductase or those into which nitrate reductase can be introduced by recombinant DNA technology, which comprises culturing the microorganism in a medium containing nitrate, 3-amino-L-tyrosine (3-AT) and luminol under suitable metabolic conditions, transferring the medium to a microtiter plate or tube coated with antibody or an antiligand to which the microorganism would specifically bind, washing the plate or tube and activating luminescence.
Further, there is provided a method for producing diazomelanin (DM) which comprises culturing in a medium containing nitrate and 3-amino-L-tyrosine (3-AT) under suitable metabolic conditions, a microorganism containing nitrate reductase.
Yet further, there is provided a method for directly detecting microorganisms containing nitrate reductase or those into which nitrate reductase can be introduced by recombinant DNA technology, which comprises culturing the microorganism in a medium containing nitrate and 3-amino-L-tyrosine (3-AT) under suitable metabolic conditions, transferring the medium to a microtiter plate or tube coated with antibody or an antiligand to which the microorganism would specifically bind, washing the plate or tube, adding luminol and activating luminescence.
REFERENCES:
patent: 4283490 (1981-08-01), Plakas et al.
patent: 5003050 (1991-03-01), Kiel et al.
patent: 5089395 (1992-02-01), Snyder et al.
"Melanin Production. . . ", della-Croppa et al. Biotechnology, vol. 8, Jul. 1990, pp. 634-638.
"Isolation and Characterization. . . ", Fernandez et al. Proc. Natl. Acad. Sci. USA, vol. 86, Sep. 1989, pp. 6449-6453.
"Biochemical and Immonological. . . ", Iobbi et al. EOR. J. Biochem, 168, 451-459 (1987).
"Stable Nuclear. . . ", Kindle et al. J. Cell Biology, vol. 109, 2589-2601 (1989).
Kiel et al, "interactions of the antioxidants 3-amino-L-tyrosine and diazoluminomelanin with cytochrome b of HL-60 cells", Free Radical Biol. Med. 9(supp.1) : 15. (1990).
Chaudhry et al., "Cytochrome b from E Scherichia coli Nitrate Reductase,"JBC, 258 (9):5819-5827. (1983).
Holwitt Eric A.
Kiel Johnathan L.
Parker Jill E.
Schwertner Harvey A.
Bricker Charles E.
Kundert Thomas L.
LeGuyader John L.
The United States of America as represented by the Secretary of
LandOfFree
Biosynthesis of diazomelanin and diazoluminomelanin and methods does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Biosynthesis of diazomelanin and diazoluminomelanin and methods , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Biosynthesis of diazomelanin and diazoluminomelanin and methods will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-861078