Biosynthesis of amorpha-4,11-diene

Details Biosynthesis of amorpha-4,11-diene Biosynthesis of amorpha-4,11-diene

2007-03-20

2007-03-20

Saidha, Tekchand (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

C435S041000, C435S183000, C435S194000, C435S232000, C435S252300, C435S320100, C536S023200

Reexamination Certificate

active

10411066

ABSTRACT:
Methods for synthesizing amorpha-4,11-diene synthase from isopentenyl pyrophosphate are provided. A first method comprises introducing into a host microorganism a plurality of heterologous nucleic acid sequences, each coding for a different enzyme in the mevalonate pathway for producing isopentenyl pyrophosphate. Amorpha-4,11-diene synthase is then produced using an optimized amorpha-4,11-diene synthase gene. The invention also provides nucleic acid sequences, enzymes, expression vectors, and transformed host cells for carrying out the methods.

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National Science Foundation Award Abstract No. 9911463.

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