Biospecific assay method

Details Biospecific assay method Biospecific assay method

2007-03-20

2007-03-20

Swartz, Rodney P (Department: 1645)

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is organic

C435S006120, C435S007100, C435S007500, C435S007920, C435S007950, C435S962000, C435S968000, C435S973000, C436S518000, C436S523000, C436S531000, C436S800000, C436S814000, C436S818000, C436S825000, C436S533000

Reexamination Certificate

active

08487623

ABSTRACT:
This invention relates to a biospecific assay method, in which microparticles coated with the bioaffinity reactant A binding the analyte to be assayed; the sample to be analyzed, and the labelled bioaffinity reactant B are mixed. After the binding reaction the signal strength from the labelled bioaffinity reactant B bound to the microparticles is quantitated for the determination of the concentration of the analyte in the sample. According to the invention, such an amount of sample and microparticles is used in the assay that after binding of the analyte of the sample to the said amount of microparticles, each individual microparticle will emit such a signal strength as to allow the measurement of the analyte concentration over the whole range of typical analyte concentrations, and the signal strength from each microparticle is measured separately.

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Bush et al., “Solid Phase Time-Resolved Fluorescence Detection of Human Immuno-deficiency Virus Polymerase Chain Reaction Amplification Products,” ANAL. Biochem 202:146-151 (1992).
Christopoulous et al., “Ultrasensitive Time-Resolved Fluorescence Method for α-Fetoprotein,” Clin. Chem. 36:1497-1502 (1990).
Ekins et al., “Multianalyte Microspot Immunoassay—Microanalytical Compact Disk of the Futute” Clin. Chem. 37:1955-1967 (1991).
Frengen et al., “Homogeneous Immunofluorometric Assays of α-Fetoprotein with Macroporous Monosized Particles and Flow Cytometry,” Clin. Chem. 39:2174-2181 (1993).

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