Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1997-06-18
1999-11-16
Ceperley, Mary E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
436501, 436512, 436527, 436532, 436534, 436823, 436828, C07K 1600, G01N 33543, G01N 33547
Patent
active
059860669
ABSTRACT:
A biologically recognizing layer on a solid phase consisting of biologically recognizing molecules comprising regions which recognize a substance to be analyzed and regions which do not recognize a substance to be analyzed.
The biologically recognizing molecules are aligned on a suitably modified surface by means of molecular regions which do not recognize the substance to be analyzed. The biologically recognizing molecules are cross-linked with the aligning surface and are consequently covalently altered. The molecular regions recognizing the substance to be analyzed are not altered by the covalent bonding and retain their bonding activity.
The layer is produced in a novel two-stage method. In the first step, the biologically recognizing molecules, the aligning molecules and carrier molecules are adsorbed. In the second step, the molecules are covalently anchored by cross-linking. Cross-linking is brought about by photolytic activation of a water soluble reagent bonded to the carrier molecules.
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Foder, et al., Angewandte Chemie, 104:801 (1992) "Photolithographische Immobilisierung von Biopolymeren auf festen Tragern".
Sanger, et al., Bioconjugate Chem, 3:308 (1992) "Light-induced Coupling of Aqueos-Soluble Proteins to Liposomes Formed from Carbene Gen. Phospholipids".
Chong, et al., Jour. of Biological Chem., 256:5064-5070 (1981) "A New Heterobifunc. Cross-linking Reagent for the Study of Biol. Interactions between Proteins".
Ueno, et al., Biochem. Biophys. Res. Commun., 191:2, pp. 701-708 (1993).
Barner Richard
Huber Walter
Hubscher Josef
Hurst Jurg
Schlatter Daniel
Ceperley Mary E.
Harris Brent A.
Roche Diagnostics Corporation
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