Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1995-06-07
1999-12-21
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
424 941, 424 946, 424 9463, 4241591, 4241641, 4241781, 4241791, 435 71, 435 19, 435 23, 435 41, 435109, 435174, 435195, 435198, 435212, 435218, 4352871, 4352872, 4352891, 435817, 436518, 514 2, 530402, 530413, 530810, C12Q 134, C12N 1100, C12N 914, C12M 134
Patent
active
060047682
ABSTRACT:
Proteins such as enzymes and antibodies are immobilized by crosslinking crystals of the proteins such as microcrystals having a cross-section of 10.sup.-1 mm or less with a multifunctional crosslinking agent. The crosslinked protein crystals may be lyophilized for storage. Crystals of an enzyme such as thermolysin, elastase, asparaginase, lysozyme, lipase or urease may be crosslinked to provide crosslinked enzyme crystals that retain at least 91% activity after incubation for three hours in the presence of a concentration of Pronase.TM. that causes the soluble uncrosslinked form of the enzyme to lose at least 94% of its initial activity under the same conditions. A preferred Pronase.TM.:enzyme ratio is 1:40. Crosslinked enzyme or antibody crystals may be used in an assay, diagnostic kit or biosensor for detecting an analyte, in an extracorporeal device for altering a component of a fluid, in producing a product such as using crosslinked thermolysin crystals to produce aspartame, in separating a substance from a mixture, and in therapy.
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Navia Manuel A.
St. Clair Nancy L.
Haley, Jr. James F.
Naff David M.
Pierri Margaret A.
Vertex Pharmaceuticals Inc.
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