Chemistry: electrical and wave energy – Apparatus – Electrolytic
Patent
1992-03-02
1993-03-09
Niebling, John
Chemistry: electrical and wave energy
Apparatus
Electrolytic
204412, 204416, 20415312, G01N 2726
Patent
active
051924153
ABSTRACT:
The biosensor of the present invention quantifies a substrate contained in a sample liquid by reducing electron acceptors using electron generated in a reaction of the substrate to enzyme and then by measuring the reduced amount of the electron acceptors electrochemically. The biosensor has an electrical insulating substrate, an electrode system including at least a working electrode and a counter electrode, a reaction layer including the enzyme provided on the electrode system and a hydrogen ion concentration control layer, and the reaction layer is in contact with the electrode system. According to the present invention, the hydrogen ion concentration in the sample liquid can be made most appropriate in accordance with the type of the enzyme contained in the reaction layer, without the adjustment of the hydrogen ion concentration in the sample liquid beforehand. Thus, the specific substrate contained in the sample liquid can be easily quantified with accuracy and speed.
REFERENCES:
patent: 4897173 (1990-01-01), Nankai et al.
patent: 5120420 (1992-06-01), Nankai et al.
Nankai Shiro
Yoshioka Toshihiko
Bell Bruce F.
Matsushita Electric - Industrial Co., Ltd.
Niebling John
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