Biosensor including chemically modified enzymes

Details Biosensor including chemically modified enzymes Biosensor including chemically modified enzymes

1994-03-04

1996-08-06

Beisner, William H.

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

435817, 204403, C12M 140, C12M 134

Patent

active

055433260

ABSTRACT:
Oxidoreductases are chemically modified so as to change their net charge at a given pH. Through such modification the adsorption of the enzymes on surfaces, their retention in similarly or oppositely charged polymer membranes and their binding with similarly or oppositely charged macromolecules, particularly redox macromolecules, is controlled. The modified enzymes, of which modified recombinant glucose oxidase is an example, are useful in biosensors, e.g. for glucose; as marker enzymes of antigens or antibodies in immunosensing; and as labels of nucleotide sequences in probes, e.g. for DNA nucleotide sequences.

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