Biosensor device and method

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 71, 435 72, 435 721, 435 75, 435 792, 435 732, 435 737, 422 8201, 422 8202, 422 8203, 427 211, 427 213, 204403, 204416, 436500, 436501, 436517, 436518, 436525, 436807, G01N 2700, G01N 33557, A61L 300, C12Q 168

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061300373

ABSTRACT:
A biosensor apparatus for detecting a binding event between a ligand and receptor. The apparatus includes an electrode substrate coated with a high-dielectric hydrocarbon-chain monolayer, and having ligands attached to the exposed monolayer surface. Binding of a receptor to the monolayer-bound ligand, and the resultant perturbation of the monolayer structure, causes ion-mediated electron flow across the monolayer. In one embodiment, the monolayers have a coil-coil heterodimer embedded therein, one subunit of which is attached to the substrate, and the second of which carries the ligand at the monolayer surface.

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