Chemistry: electrical and wave energy – Apparatus – Electrolytic
Patent
1997-04-24
2000-12-26
Tung, T.
Chemistry: electrical and wave energy
Apparatus
Electrolytic
436164, 436908, G01N 2726, G01N 2100
Patent
active
061653357
ABSTRACT:
A biosensor apparatus for detecting a binding event between a ligand and receptor. The apparatus includes a biosensor surface and surface-bound two-subunit heterodimer complexes composed of first and second, preferably oppositely charged peptides that together form an .alpha.-helical coiled-coil heterodimer. The first peptide is attached to the biosensor surface, and the second peptide carries the ligand, accessible for binding by a ligand-binding agent. Binding of anti-ligand binding agent to the surface-bound ligand is detected by a suitable detector. A ligand-specific biosensor surface can be readily prepared from a universal template containing the first charged peptide, by addition of a selected ligand attached to the second peptide.
REFERENCES:
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Monera, O.D., et al., "Electrostatic interactions control the parallel and antiparallel orientation of .alpha.-helical chains in two-stranded .alpha.-helical coiled-coils," Biochemistry 33(13) (1994) F3862-3871.
O'Shea, E.K., et al., "Peptide `velcro.RTM.`: design of a heterodimeric coiled coil," Current Biology 3(10) (1993) F658-667.
Zhou, N.E., et al., "The two-stranded .alpha.-helical coiled-coil is an ideal model for studying protein stability and subunit interactions," Biopolymers 32 (1992) F419-426.
Hodges Robert S.
Irvin Randall T.
Lennox R. Bruce
Dehlinger Peter J.
Heinkel Gregory L.
Noguerola Alex
Pence and McGill University
Tung T.
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