Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Multi-enzyme system
Patent
1990-08-27
1993-01-05
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Multi-enzyme system
204403, 435 14, 435177, 435178, 435180, 435182, 435288, 435817, C12N 1118, C12N 1102, C12M 140
Patent
active
051770121
ABSTRACT:
A biosensor for the determination of glucose and fructose concentrations is provided. The biosensor is produced by a method which comprises treating Zymomonas mobilis whole cells with an organic solvent such as xylene and n-butanol, immobilizing the treated whole cells onto a support selected from the group consisting of gelatin, collagen, agarose, cellophane and polyacrylamide to give an immobilized whole cell enzyme membrane and adhering the membrane to the surface of a pH electrode to give the biosensor. The resulting biosensor is capable of determning glucose and fructose in high concentrations such as 5 g/L and 50 g/L, respectively. Invertase can be immobilized with the whole cells to provide a biosenser for the determination of sucrose.
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Ikuo Satoh, et al., "Enzyme Electrode for Sucrose," Biotechnology and Bioengineering, vol. XVIII (1976), pp. 269-272.
Jean-Louis Romette et al., "Glucose-Oxidase Electrode, Measurement of Glucose in Samples Exhibiting High Variability in Oxygen Content," Clinica Chimica Acta, 95 (1979), pp. 249-253.
Kim Hak-Sung
Park Je-Kyun
Korea Advanced Institute of Science & Technology
Naff David M.
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