Biosensor containing a biochemical substance immobilized on a la

Details Biosensor containing a biochemical substance immobilized on a la Biosensor containing a biochemical substance immobilized on a la

1993-03-22

1995-02-14

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

204403, 435176, 435177, 435181, 435288, 435817, 436531, 436532, 530815, 530816, C12N 1108, C12N 1106, C12M 140, G01N 33545

Patent

active

053895347

ABSTRACT:
A biosensor is prepared having a selective detection system containing a biochemical substance such as an enzyme immobilized by reaction with epoxy groups of an olefinic-unsaturated, epoxyfunctional polyether. Prior to immobilization, the polyether is applied to a carrier and crosslinked by treatment with high-energy radiation or peroxide to form a layer. After reacting the biochemical substance with epoxy groups, non-reacted epoxy groups are reacted with a compound containing an amino group and/or a carboxyl group such as an amino acid. Before immobilizing of the biochemical substance and after crosslinking, the polyether may be hydrophilized by reacting some of the epoxy groups with a hydrophilic compound such as an amino acid.

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