Biosensor containing a biochemical substance immobilized on a la

Details Biosensor containing a biochemical substance immobilized on a la Biosensor containing a biochemical substance immobilized on a la

1993-03-22

1995-04-18

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435 12, 435 14, 435 25, 435 26, 435 27, 435176, 435288, 204403, 528 25, C12M 1108, C12M 140, G01N 2726, C08G 7704

Patent

active

054078180

ABSTRACT:
A biosensor is prepared having a selective detection system containing a biochemical substance such as an enzyme immobilized on an olefinic-unsaturated, epoxyfunctional polysiloxane. Prior to immobilization of the biochemical substance, the polysiloxane is applied as a layer to a carrier and cross-linked by treatment with high-energy radiation. A biochemical substance is reacted with epoxy groups of the cross-linked polysiloxane. Any non-reacted epoxy groups are reacted with a compound containing an amino group, a carboxyl group or an amino group and a carboxyl group to stabilize. After cross-linking and before reacting of the biochemical substance, the cross-linked polysiloxane can be hydrophilized by reacting some of the epoxy groups with a hydrophilic compound containing a reactive group.

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