Biosensor carrying redox enzymes

Details Biosensor carrying redox enzymes Biosensor carrying redox enzymes

2006-03-28

2006-03-28

Olsen, Kaj K. (Department: 1753)

Chemistry: electrical and wave energy

Apparatus

Electrolytic

C204S403010, C429S002000, C427S002120

Reexamination Certificate

active

07018518

ABSTRACT:
The present invention concerns an electrode carrying immobilized redox enzymes such that electric charge can flow between an electron mediator group to the enzyme cofactor by the use of boronic acid or a boronic acid derivative that acts as a linker moiety between the cofactor and the electron mediator group. The invention also concerns devices and systems that make use of the electrode of the invention, such as bio-sensors and fuel cells, the electrode being one of the components thereof.

REFERENCES:
patent: 6241863 (2001-06-01), Monbouquette
Habermuller et al. “Electron-Transfer Mechanisms in Amperometric Biosensors”,Anal. Chem.,366:560-568, 2000.
Heller, “Electrical Wiring of Redox Enzymes”,Acc. Chem. Res., 23:128-134, 1990.
Willner et al. “Electrical Contact of Redox Enzyme Layers Associated with Electrodes: Routes to Amperometric Biosensors”,Electroanalysis,9 (13):965-977, 1997.
Chen et al., “A Miniature Biofuel Cell”,J. Am. Chem. Soc.,123:8630-8631, 2001.
Katz et al., “Short Communication: A Non-Compartmentalized Glucose | O2Biofuel Cell by Bioengineered Electrode Surfaces”,J. Electroanal. Chem.,479:64-68, 1999.
Willner et al., “Integration of a Reconstituted de Novo Synthesized Hemoprotein and Native Metalloproteins with Electrode Supports for Bioelectronic and Bioelectrocatalylic Applications”,J. am. Chem. Soc.,121:6455-6468, 1999.
Willner et al., “Mediated Electron Transfer in Glutathione Reductase Organized in Self-Assembled Monolayers on Au-Electrodes”,J. Am. Chem. Soc., 114:10965-10966, 1992.
Willner et al., “Development of Novel Biosensor Enzyme Electrodes: Glucose Oxidase Multilayer Arrays Immobilized onto Self-Assembled Monolayers on Electrodes”,Adv. Mater., 5 (12):912-915, 1993.
Gregg et al., “Redox Polymer Films Containing Enzymes. 1. A Redox-Conducting Epoxy Cement: Synthesis, Characterization, and Electrocatalytic Oxidation of Hydroquinone”,J. Phys. Chem.,95:5970-5975, 1991.
Cosnier et al., “Amperometric Detection of Nitrate via a Nitrate Reductase Immobilized and Electrically Wired at the Electrode Surface”,Anal. Chem.,66:3198-3201, 1994.
Badia, et al., “Intramolecular Electron-Transfer Rates in Ferrocene-Derivatized Glucose Oxidase”,J. Am. Chem. Soc.,115 (16):7053-7060, 1993.
Katz et al., “Glucose Oxidase Electrodes via Reconstruction of the Apo-Enzyme: Tailoring of Novel Glucose Biosensors”,Anal. Chim. Acta,385:45-58, 1999.
Buckmann et al., “Synthesis of N6-(2-Aminoethyl)-FAD, N6-(6-Carboxyhexyl)-FAD, and Related Compounds”, in McCormick, D.B. (Ed.),Methods in Enzymology: Vitamins and Coenzymes, Academic Press,280(1):360, 1997.
James et al., “Saccharide Sensing with Molecular Receptors Based on Boronic Acid”,Angew. Chem. Int. Ed. Engl.,35:1911-1922, 1996.
Lorand et al., “Polyol Complexes and Structure of the Benzeneboronate Ion”,J. Org. Chem.24, 769-88, 1959.
Katz et al., “Kinetic Separation of Amperometric Responses of Composite Redox-Active Monolayers Assembled onto Au Electrodes: Implications to the Monolayers' Structure and Composition”,Langmuir13: 3364-3373, 1997.
Allvarez-Icazan et al, “Observation of Direct Electron Transfer from the Active Center of Glucose Oxidase to a Graphite Electrode Achieved Through the Use of Mild Immobilization”, Biochemistry and Bioenergetics, vol. 33, 1994, pp. 191-199.
Frew, J.E., and H.A.O. Hill, “Electron-Transfer Biosensors”, Phil. Trans. R. Soc. Lond., vol. B316, 1987, pp. 95-106.
Narasimhan, Krishna, and Lemuel B. Wingard, Jr., “Enhanced Direct Electron Transport with Glucose Oxidase Immobililzed on (Aminophenyl)boronic Acid Modified Glassy Carbon Electrode”, Anal. Chem., vol. 58, 1986. pp. 2984-2987.
Willner et al, “Electrical Wiring of Glucose Oxidase by Reconstitution of FAD-Modified Monolayers Assembled onto Au-Electrodes”, J. Am. Chem. Soc., vol. 118, 1996, pp. 10321-10322.
Zayats et al, “Electrical Contacting of Flavoenzymes and NAD(P)=-Dependent Enzymes by Reconstitution and Affinity Interactions on Phenylboronic Acid Monolayers Associated with Au-Electrodes”, J. Am. Chem. Soc., vol. 124, 2002, pp. 14724-14735.
Zayats et al, “Electrical Contacting of Glucose Oxidase by Surface-Reconstitution of the Apo-Protein on a Relay-Boronic Acid-FAD Cofactor Monolayer”.

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