Biosensor and method for quantitative measurement of a substrate

Details Biosensor and method for quantitative measurement of a substrate Biosensor and method for quantitative measurement of a substrate

1998-09-24

1999-05-25

Leary, Louise N.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 14, 435 4, 435 8201, 4352891, 435817, 4352831, 4352852, 4352871, 422 50, 422 681, 422 8201, 422 8202, 422 8203, C12Q 126, C12Q 154, C12Q 100

Patent

active

059069210

ABSTRACT:
A biosensor is disclosed for application to a method for quantitative measurement of a substrate comprising a first step of causing a substrate contained in a sample to react with an oxidoreductase specific to the substrate in the presence of an electron mediator in oxidized state, a second step of applying a potential to a working electrode for reducing the electron mediator in oxidized state that remains not reduced in the first step, and a third step of measuring a reduction current flowing across the working electrode and a counter electrode. The biosensor comprises an electrically insulating base plate, an electrode system having a working electrode and a counter electrode formed on the base plate, and a reaction layer which is formed on or in the vicinity of the electrode system and contains at least an oxidoreductase and an electron mediator, the counter electrode including at least a reductant of a redox compound or an electrolytically oxidizable metal.

REFERENCES:
patent: 5120420 (1992-06-01), Nankai et al.
patent: 5264103 (1993-11-01), Yoshioka et al.
patent: 5582697 (1996-12-01), Ikeda et al.
patent: 5650062 (1997-07-01), Ikeda et al.
patent: 5651869 (1997-07-01), Yoshioka et al.

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