Biosensor and method for operating the latter

Details Biosensor and method for operating the latter Biosensor and method for operating the latter

2005-01-27

2010-12-14

Mosher, Mary E (Department: 1648)

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

C204S403010, C435S005000, C435S007100, C435S025000

Reexamination Certificate

active

07851202

ABSTRACT:
A biosensor for detecting an antigen using an antigen/antibody coupling includes: a silicon substrate, at least one interdigital electrode pair structure that is located on the silicon substrate, the electrode pair being interspaced at a maximum distance of 1.0 μm; a counter-electrode on the silicon substrate; a reference electrode; a first layer of protein, covering at least the interdigital electrode structure; a selective second protein layer applied to the first layer and containing a capture antibody selected specifically with respect to the antigen of interest and to which the antigen can be coupled. A sensor signal can be read on the interdigital electrode structure, if the antigen is coupled to the capture antibody by way of a sample to be analyzed that comes into contact with the biosensor and a redox reactive molecule is enzymatically released on the sensor surface by an enzyme-marked detection antibody likewise coupled to the antigen.

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