Biosensor and interface membrane

Chemistry: electrical and wave energy – Apparatus – Electrolytic

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

204415, 204418, 205778, 4352871, 435817, 4352879, G01N 2726

Patent

active

056245378

ABSTRACT:
A regenerable biosensor probe adapted for positioning in a bioreactor comprises a selectively permeable interface membrane, a porous protein-receiving matrix adjacent to the interface membrane, an indicating electrode, an inlet conduit through which fresh protein conjugate may flow to the protein-receiving matrix, and an outlet conduit through which spent protein conjugate may be removed from the protein-receiving matrix. A selectively permeable interface membrane, which may be used in a biosensor system to separate biochemical, optical or other processes from an analyte matrix comprises a supporting mesh, a perfluorosulfonic acid polymer impregnated substrate and a homogenous film of perfluorosulfonic acid polymer.
A method of preparing this interface membrane comprises fixing a substrate on to a supporting mesh to form a substrate membrane, casting a perfluorosulfonic and polymer on the substrate membrane and curing the product to so formed.

REFERENCES:
patent: 5165407 (1992-11-01), Wilson et al.
patent: 5254223 (1993-10-01), Josowicz et al.
patent: 5326449 (1994-07-01), Cunningham
Koopal, C.G.J. and Nolte, R.J.M., "Highly stable first-generation biosensor or glucose utilizing latex particles as the enzyme-immobilizing matrix," Enzyme and Microbial Technology, 16(5):402-408 (1994). No month available.
Soldatkin, A.P. et al., "Glucose sensitive conductometric biosensor with additional Nafion membrane: reduction on influence of buffer capacity on the sensor response and extension of its dynamic range," Analytica Chimica Acta, 288(3):197-203 (1994). No month available.
Vaidya, R. and Wilkins, E., "Application of Polytetrafluoroethylene (PTFE) Membranes to Control Interference Effects in a Glucose Biosensor," Biomedical Instrumentation & Technology, 27(6):486-494 (1993). No month available.
Rishpon, J. et al., "Amperometric Glucose Sensors Based on Glucose Oxidase Immobilized in Nafion," Electroanalysis, 6(1):17-21 (1994). No month available.
Wang, J. and Wu, H., "Permselective lipid-poly(o-phenylenediamine) coatings for amperometric biosensing of glucose," Analytica Chimica Acta, 283(2):683-688 (1993). No month available.
Uhegbu, C.E. et al., "Initial Studies of a New Approach to the Design and Use of Enzyme-Based Reactor/Sensor Systems: Amperometric System for Glucose," Analytical Chemistry, 65(18):2443-2451 (1993). No month available.
Rohde, E. et al., "Development of a flow-through electrochemical detector for glucose based on a glucose oxidase-modified microelectrode incorporating redox and conducting polymer materials," Analytica Chimica Acta, 278(1):5-16 (1993). No month available.
Koshy, A. et al., "Elimination of electrochemically-active interferents in amperometric biosensor measurements; application to on-line monitoring of glucose and glutamate," Analytical Letters, 26(5):831-849 (1993). No month available.
Bradley, J. and Schmid, R.D., "Optimisation of a biosensor for in situ fermentation monitoring of glucose and concentration," Biosensors and Bioelectronics, 6:669-674 (1991). No month available.
Brooks, S.L. et al., "Development of an On-line Glucose Sensor for Fermentation Monitoring," Biosensors, 3:45-56 (1987-88). No month available.
Cleland, N. and Enfors, S.-O., "Monitoring glucose consumption in an Escherida coli cultivation with an enzyme electrode," Anal. Chim. Acta, 163:281-285 (1984). No month available.
Cleland, N. and Enfors, S.-O., "Control of Glucose-Fed Batch Cultivations of E. coli by Means of an Oxygen Stabilized Enzyme Electrode," Eur. J. Appl. Microbio. Biotechnol., 18:141-147 (1983). No month available.
de Alwis, U. and Wilson, G.S., "Strategies for the reversible immobilization of enzymes by use of biotin-bound anti-enzyme antibodies," Talanta, 36(1/2):249-253 (1989). No month available.
Enfors, S.-O., "Oxygen stabilized enzyme electrode for D-glucose analysis in fermentation broths," Enzyme Microbiol. Technol., 3:29 (1981). No month available.
Foulds, N.C. and Lowe, C.R., "Enzyme Entrapment in Electrically Conducting Polymers," J. Chem. Soc., Faraday Trans., I., 82:1259-1264 (1986). No month available.
Harrison, D.J. et al., "Characterization of Perfluorosulfonic-Acid Polymer Coated Enzyme Electrodes and a Miniaturized Integrated Potentiostat for Glucose Analysis in Whole Blood," Anal. Chem., 60:2002-2007 (1988). No month available.
Lee, J.-S. et al., "A new glucose sensor using microporous enzyme membrane," Sensors and Actuators B., 3:215-219 (1991). No month available.
Phelps, M.R. et al., "Technology for Regenerable Biosensor Probes Based on Enzyme-Cellulose Binding Domain Conjugates," Biotechnol. Prog., 10:433-440 (1994). No month available.
Renneberg, R. et al., "Enzyme sensor-FIA system for on-line monitoring of glucose, lactate and glutamine in animal cell cultures," J. Biotech., 21:173-186 (1991). No month available.
Wang, J., "Permselective Coatings for Amperometric Biosensing," In Biosensors and chemical sensors: optimizing performance through polymeric materials, Eddman, P.G. and Wang, J., Eds., Symposium series 487:125-132 (ACS: New Mexico State University, 1992). No month available.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Biosensor and interface membrane does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Biosensor and interface membrane, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Biosensor and interface membrane will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-703101

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.