Bioremediation of nitrogenous contaminants

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Reexamination Certificate

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Reexamination Certificate

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06936456

ABSTRACT:
A novel process for the remediation of nitrogenous energetic materials such as 1,3,5,-trinitro-1,3,5-triazine (RDX) which can be used in situ on contaminated media is provided. The process comprises the bioremediation by one or more miccroorganisms capable of metabolizing the energetic materials. Examples of such microorganisms includeRhizobium rhizogenes, Burkholderiasp., andCladosporium cladosporioides(ATCC 66669). Strains of these microorganisms have been deposited. The strain designated A1 has been deposited asRhizobium rhizogenesBL (ATCC PTA-4110) and the strain designated C8 has been deposited asBurkholderiasp. (ATCC PTA-4111) Additionally, with the addition of a carbon source, such as a sugar, the process can totally degrade the energetic materials in two to three days.

REFERENCES:
patent: 5736669 (1998-04-01), Thomas et al.
patent: 6066772 (2000-05-01), Hater et al.
patent: 6274368 (2001-08-01), Nicklin et al.
patent: 6334395 (2002-01-01), Badger et al.
patent: 6668725 (2003-12-01), Badger et al.

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