Bioreactor system and method for probing toxic materials

Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing

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435808, 435849, C12M 134

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active

061330213

ABSTRACT:
A bioreactor system of probing toxic materials using microorganisms comprises two reactors. A first-step reactor serves as a microorganism reservoir in which the microorganisms are continuously cultured in a constant phase and a second-step reactor, provided with fiber optics, offers a place in which the microorganisms meet the toxic materials for the first time. Since the microorganisms are so genetically recombinant as to luminesce upon reaction with toxic materials, light is generated in the second-step reactor and, then, transmitted along the fiber optics to a luminometer where the change in the intensity of the light is monitored over time. The microorganisms can be stably provided from the reservoir, so that it is possible to continuously monitor the light intensity and thus, to trace the pollution of the toxic materials. The toxic materials may come from any of the sources including rivers, waste water, sewage, agricultural and industrial water, household water, tap water, atomic power plants, etc.

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K. W. Schramm, A. Kaune, B. Beck, W. Thumm, A. Behechti, A Kettrup, P. Nickolova; Acute Toxicities of Five Nitromusk Compounds in Daphnia, Algae and Photoluminescent Bacteria; Wat. Res. vol. 30, No. 10, pp. 2247-2250; Apr. 1996.
S.A.L.M. Kooijman, J.J.M. Bedaux; Analysis of Toxicity Tests on Daphnia Survival and Reproduction; Wat. Res. vol. 30, No. 7, pp. 1711-1723; Feb. 1996.
Leonard I. Sweet, David F. Tracers, Peter G. Meier; Chronic Toxicity Evaluation of Wastewater Treatment Plant Effluents with Bioluminescent Bacteria: A Comparison with Invertebrates and Fish; Environmental Toxicology and Chemistry, vol. 16, No. 10, pp. 2187-2189; Apr. 11, 1997.
Mikio Kikuchi, Meiko Wakabayashi; Monitoring the Biological Effects of Chemicals in River Water using Daphnia magna; pp. 627-633; 1997.

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