Bioreactor system

Details Bioreactor system Bioreactor system

1991-02-11

1992-01-14

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Apparatus

Differentiated tissue perfusion or preservation apparatus

435240242, 435311, 435313, 21032179, C12M 306

Patent

active

050810359

ABSTRACT:
A system for continuous perfusion culturing of anchorage-dependent and anchorage-independent mammalian cell lines, inter alia, wherein in vivo capillary bed conditions are simulated by providing a bioreactor having a growth chamber wherein the cells are grown between a multitubular array comprising at least three functionally separate, inert non-degradable and structurally strong tubes or sets of tubes. A constant nutrient gradient is maintained along the entire length of the tubes by perfusing medium through the tubes at a flow rate which is sufficient to expose all areas of the chamber to fresh medium by convective forces rather than diffusion. Oxygen transfer and removal of toxic wastes is improved by the invention herein. In a preferred embodiment, porous inert expanded Teflon tubes are employed having an inner diameter of approximately between 1 and 2 millimeters.

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