Bioreactor for mammalian cell growth and maintenance

Details Bioreactor for mammalian cell growth and maintenance Bioreactor for mammalian cell growth and maintenance

1995-06-07

1997-11-18

Beisner, William H.

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

4352972, 4352975, C12M 300

Patent

active

056886870

ABSTRACT:
A bioreactor for the ex vivo maintenance and growth of mammalian cells includes a disposable, self contained cell cassette, a system manager, an incubator unit matable with a plurality of the cassettes and a processor unit. The cell cassette includes a substantially circular cell growth chamber defined between a substantially planar cell bed and a gas permeable, liquid impervious membrane. A media inlet enters the cell growth chamber communicates at a radially central portion of the cell bed. A plurality of media outlets are radially outwardly spaced from the media inlet by a distance sufficient for cell growth to occur between the inlet and the outlets. The outlets are substantially equiangularly circumferentially spaced about the cell growth chamber.

REFERENCES:
patent: 4654308 (1987-03-01), Safi et al.
patent: 5153131 (1992-10-01), Wolf et al.
patent: 5416022 (1995-05-01), Amiot
patent: 5459069 (1995-10-01), Palsson et al.
Crouch et al. "The Adhesion of Animal Cells to Surfaces: . . . " J. Chem. Tech. Biotech. vol.35B (1985)pp. 273-281.

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