Bioreactor for culturing and propagating cells with a woven moti

Chemistry: molecular biology and microbiology – Apparatus – Bioreactor

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Details

4352891, C12M 304, C12M 114, C12M 102

Patent

active

055653618

ABSTRACT:
A bioreactor, motionless mixing element with attached cells, and method for the enhanced cultivation and propagation of cells in a bioreactor method, and which bioreactor comprises a housing and contains within the housing a motionless mixing element, the attachment of cells to the mixing element and a nutrient composition permitting the cell growth and division of the attached cells, and which motionless mixing element and the method and bioreactor comprise a porous, fibrous sheet material; for example, of a corrugated or knitted, woven wire material, such as stainless steel or titanium, and of selected dimensions as to the fiber diameter and height, to provide a maximum surface area for the attachment of the cells to be cultivated, with the ratio of the fibrous, geometric surface area of the porous, fibrous sheet material to that of a sheet material of the same shape being greater than about 1.0; for example, 1.5, and typically, for example, having a surface area for cell growth of about 2000 to 3000 M.sup.2 /M.sup.3.

REFERENCES:
patent: 4296204 (1981-10-01), Grabner
patent: 5476783 (1995-12-01), Mutsakis et al.
Paul, "Design and Scaleup of an Anchorage-Dependent Mammilian Cell Bioreactor.", Annals New York Academy of Sciences, 589 (Biochem. Eng. 6), pp. 642-649, 1990.

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