Bioreactor for cell culture

Details Bioreactor for cell culture Bioreactor for cell culture

2005-09-13

2005-09-13

Ketter, James (Department: 1636)

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

C435S399000

Reexamination Certificate

active

06943008

ABSTRACT:
A bioreactor for cell culture includes a chamber having three channels therethrough for conducting fluids, the three channels including an upper channel, a middle channel, and a lower channel, each the channel having an inlet and an outlet approximately opposite along the channel from the inlet. First and second cell support scaffolds are positioned within the chamber, each scaffold comprising at least one three-dimensional porous matrix containing non woven fibrous polyethylene terephthalate, the first scaffold being positioned within the chamber between the upper channel and the middle channel, and the second scaffold being positioned within the chamber between the middle channel and the lower channel.

REFERENCES:
Rainger et al., Journal of Immunological Methods, vol. 225 (2001), pp. 73-82.
Pazzano et al.; “Comparison of Chondrogenis in Static and Perfused Bioreactor Culture;” Biotechnol. Prog vol. 16; No. 5; pp. 893-896; 2000.
Ingram et al.; “Three-Dimensional Growth Patterns of Various Human Tumor Cell Lines in Simulated Microgravity of A NASA Bioreactor;” In Vitro Cell. Dev. Biol.—Annual ; pp. 459-466; Jun. 1997.
Saini et al.; “Concentric Cylinder Bioreactor For Production of Tissue Engineered Cartilage: Effect of Seeding Density and Hydrodynamic Loading on Construct Development;” Biotechnol. Prog. 2003 vol. 19; (2003); pp. 510-521.
Cabrita et al.; “Hematopoietic stem cells: from the bone to the bioreactor;” Trends in Biotechnology; TIBTEC 45; 2003.
Bannu et al.; “Cytokine-Augumented Culture of Haematopoietic Progenitor Cells in a Novel Three-Dimensional Cell Growth Matrix;” Cytokine; vol. 13, No. 6; Mar. 21, 2000; pp. 349-358.
Blythe et al.; “Seroprevalence of antibodies to Sarcocystis neurona in horses residing in Oregon;” Journal of the American Veterinary Medical Association; vol. 210, No. 4, Feb. 15,. 1997; pp. 525-527.
Bagley et al., “Extended culture of miltipotent hematopoietic progenitors without cytokine augmentation in a novel three-dimensional device;” Experimental Hematoloty 27 (1999); pp. 496-504.
Obradovic et al.; “Gas Exchange is Essential for Bioreactor Cultivation of Tissue Engineered Cartilage;” Biotechnology and Bioengineering; vol. 63, No. 2, Apr. 20, 1999; pp. 197-205.
Hoerstrup, MD et al.; “New Pulsatile Bioreactor for in Vitro Formation of Tissue Engineered Heart Valves;” Tissue Engineering; vol. 6, No. 1, 2000; pp. 75-78.
Halberstadt et al.; “The In Vitro Growth of a Three-Dimensional Human Dermal Replacement Using a Single-Pass Perfusion System;” Biotechnology and Bioengineering; vol. 43, No. 4, 1994; pp. 740-746.
Vunjak et al., “Dynamic Cell Seeding of Polymer Scaffolds for Cartilage Tissue Engineering;” Biotechnol. Prog. 1998, vol. 14, No. 2; pp. 193-202.
Kim, MD et al.; “Dynamic Seeding and in Vitro Culture of Hepatocytes in a Flow Perfusion System;” Tissue Engineering; vol. 6, No. 1, 2000; pp. 39-44.
Ma et al.; “Development of an In Vitro Human Placenta Model by the Cuotivation of Human Trophoblasts in a Fiber-Based Bioreactor System;” Tissue Engineering; vol. 5, No. 2; (1999); pp. 91-101.
Freed et al.; “Cuotivation of Cell-Polymer Cartilage Implants in Bioreactors;” Journal of Cellular Biochemistry vol. 51; 1993; pp. 257-264.
Sittinger; “Artificial tissues in perfusion culture;” The International Journal of Artificial Organs; vol. 20, No. 1; 1997; pp. 57-62.
Niklason; “Functional Arteries Grown In Vitro;” Science; vol. 284; Apr. 16, 1999; pp. 489-493.
Nielsen; “Bioreactors For Hematopoietic Cell Culture;” Annu. Rev. Biomed. Eng.; 1999; pp. 129-152.
Li et al., “Human Cord Cell Hematopoiesis in Three Dimensional Nonwoven Firbous Matrices: In Vitro Simulation of the Marrow Microenvironment”, Jounral of Hematotherapy & Stem Cell Research; vol. 10; 2001; pp. 355-368.
Collins et al.; “Characterization of Hematopoietic Cell Expansion, Oxygen Uptake, and Glycolysis in a Controlled, Stirred-Tank Bioreactor System;” Biotechnol. Prog. 1998 vol. 14; pp. 466-472.
Ma et al; “Oxygen Tension Influences Proliferation and Differentiation in a Tissue-Engineered Model of Pleacntal Trophoblast-Like Cells;” Tissue Engineering vol. 7; No. 5; 2001; pp. 496-506.

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