Bioreactor containing cells expressing glycosyltransferase...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process...

Reexamination Certificate

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C435S325000, C435S348000, C435S419000, C435S252300, C800S278000, C800S298000

Reexamination Certificate

active

07662583

ABSTRACT:
We describe glycosyltransferase nucleic acids and proteins isolatable fromArabidopsis thaliana, transgenic cells expressing said glycosyltransferases, and bioreactors comprising said transgenic cells together with nutrient medium for supporting the growth of said cell that includes at least one exogenous substrate which is a substrate for said glycosyltransferase, and wherein said nutrient medium does not include an exogenous supply of UDP-glucose.

REFERENCES:
patent: WO 01/59140 (2001-08-01), None
patent: WO 02/10210 (2002-02-01), None
patent: WO 02/103022 (2002-12-01), None
patent: WO 03/023035 (2003-03-01), None
patent: WO 03/023035 (2003-03-01), None
Mok et al. Genes encoding zeatin O-glycosyltransferases. (2000) Plant Growth Regulators, vol. 32, pp. 285-287.
Hefner et al. Arbutin synthase, a novel member of the NRD1beta glycosyltransferase family, is a unique multifunctional enzyme converting various natural products and xenobiotics. (2002) Bioorganic and Medicinal Chemistry; vol. 10, pp. 1731-1741.
Lacombe B. et al. The identity of plant glutamate receptors. (2001) Science; vol. 292, pp. 1486-1487.
Lazar et al. Transforming growth factor alpha: mutation of aspartic acid 47 and leucine 48 results in different biological activities. (1988) Mol. Cell. Biol.; vol. 8; pp. 1247-1252.
Hill et al. Functional analysis of conserved histidines in ADP-glucose pyrophosphorylase fromEscherichia coli. (1998) Biochem. Biophys. Res. Comm. vol. 244; pp. 573-577.
Guo et al. Protein tolerance to random amino acid change. (2004) Proc. Natl. Acad. Sci. USA; vol. 101; pp. 9205-9210.
EMBL Accession No. AB025634 (Apr. 9, 1999).
Accession No. AX372955 (Mar. 1, 2002).
EMBL Accession No. Q9LSY4 (Oct. 1, 2000).
Mok et al., “Genes Encoding Zeatin O-Glycosyltransferases,”Plant Growth Reg. 32:285-287 (2000).

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