Bioreactor and methods for producing synchronous cells

Details Bioreactor and methods for producing synchronous cells Bioreactor and methods for producing synchronous cells

2005-03-15

2005-03-15

Redding, David A. (Department: 1744)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of regulating cell metabolism or physiology

C435S372000, C435S395000, C435S401000, C435S402000, C435S297200, C435S297300, C435S298200

Reexamination Certificate

active

06867040

ABSTRACT:
Apparatus and methods are directed to a perfusion culture system in which a rotating bioreactor is used to grow cells in a liquid culture medium, while these cells are attached to an adhesive-treated porous surface. As a result of this arrangement and its rotation, the attached cells divide, with one cell remaining attached to the substrate, while the other cell, a newborn cell is released. These newborn cells are of approximately the same age, that are collected upon leaving the bioreactor. The populations of newborn cells collected are of synchronous and are minimally, if at all, disturbed metabolically.

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