Bioreactor and bioprocessing technique

Details Bioreactor and bioprocessing technique Bioreactor and bioprocessing technique

2006-05-09

2006-05-09

Redding, David (Department: 1744)

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

C435S288400, C422S082050, C356S319000, C356S320000, C356S326000, C356S246000

Reexamination Certificate

active

07041493

ABSTRACT:
The inventive bioprocessing system (and technique) relies on non-invasive optical chemical sensing technology wherein an optical excitation source excites an optical chemical sensor. The optical chemical sensor then emits luminescence or absorbs light which is measured by a detector. The luminescence emitted from the chemical sensor or the amount of light absorbed by the chemical sensor is related to the concentration of an analyte, such as oxygen. If the luminescence emitted changes, or if the amount of light absorbed changes, then the concentration of the analyte has changed. Using such a system to measure and adjust multiple parameters at one time allows one to efficiently and cost-effectively determine optimal conditions for a given cell type and/or cell environment, for example. By combining cell cultivation with optical chemical sensing technology, cultivation can be successfully and rapidly performed, controlled and monitored in small volumes in an automated, parallel fashion at less expense than current bioprocess techniques.

REFERENCES:
patent: 5266486 (1993-11-01), Fraatz et al.
patent: 5731212 (1998-03-01), Gavin et al.
patent: 5976827 (1999-11-01), Jeffrey et al.
Chang et al., “Steam-Sterilizable, Fluorescence Lifetime-Based Sensing Film for Dissolved Carbon Dioxide”, Biotechnol Prog, Mar.-Apr., 14 (2):326-331 (1998).
Bylund et al., “Influence of Scale-Up on the Quality of Recombinant Human Growth Hormone”, Biotechnol Bioeng, 69:119-128 (2000).
Kostov et al., “Low-Cost Mirobioreactor for High-Throughput Bioprocessing”, Biotechnol Bioeng, Feb. 5, 72 (3):346-352 (2001).
S.B. Bambot et al., “Lifetime-based optical sensing of pH using resonance energy transfer in solgel films”, Sensors and Actuators B 22 (1994) pp. 181-188.
Sipior et al., “A Lifetime-Based Optical CO2 Gas Sensor with Blue or Red Excitation and Stokes or Anti-Stokes Detection”, Anal Biochem, May 20, 227 (2):309-18 (1995).
Gryczynski et al., “Polarization-Based Oxygen Sensor”, Analyst, Jul., 124(7):1041-44 (1999).
Bambot et al., “Optical Oxygen Sensor Using Fluorescence Lifetime Measurement”, Adv Ep Med Biol, 361:197-205 (1994).
Chae et al., “Framework for Online Optimization of Recombinant Protein Expression in High-Cell-DensityEscherichia coliCultures using GFP-Fusion Monitoring”, Biotechnol Bioeng, Aug. 5, 69(3):275-85 (2000).
DeLisa et al., “Monitoring GFP-Operon Fusion Protein Expression during High Cell Density Cultivation ofExcherichia coliusing an On-Line Optical Sensor”, Biotechnol Bioeng, Oct. 5, 65(1) :54-64 (1999).

Affiliated with

Also associated with

Rating

Bioreactor and bioprocessing technique does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Bioreactor and bioprocessing technique, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Bioreactor and bioprocessing technique will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3562992