Bioprotectant composition, method of use and extraction process

Details Bioprotectant composition, method of use and extraction process Bioprotectant composition, method of use and extraction process

1997-10-27

1999-01-19

Krass, Frederick

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Having -c-, wherein x is chalcogen, bonded directly to...

4241951, 514492, 514499, 514505, 514720, 514733, 568324, 568325, 568334, A61K 31445, A61K 3128, A61K 3130, A61K 31075

Patent

active

058614150

DESCRIPTION:

BRIEF SUMMARY
This is a prov. appln. of Ser. No. 60-001,161, filed Jul. 14, 1995.
Curcuma longa (Fam. Zingiberaccae) or Turmeric is one of the oldest herbs in Ayurveda materia medica, and has been used in Ayurveda medicine internally as a stomach, tonic and blood purifier, and topically in the prevention and treatment of skin diseases. The significance of turmeric in medicine has changed considerably since the very recent discovery of the anti-oxidant properties of naturally occurring phenolic compounds. The same ground dried rhizome of Curcuma longa, which has been used for centuries as a spice, food preservative and a coloring agent, has been found to be a rich source of phenolic compounds or curcuminoids. There are three main curcuminoids recognized, i.e., curcumin(diferuloylmethane), curcumin(p,p-dihydroxydicinnamoylmethane).
Curcuminoids have scientifically documented anti-oxidant, anti-inflammatory, anti-bacterial, anti-fungal, antiparasitic, anti-mutagen, anti-cancer and detox properties. Their potential use in the prevention of cancer and in the treatment of infection with human immunodeficiency virus (HIV) are the subject of intensive laboratory and clinical research. Curcuminoids are recognized for their broad biological activity and safety of use. The biological activity of curcuminoids can best be described by the word "protective".
Curcumin and turmeric have been used as anti-inflammatory drugs for many years. More recently, there has been much interest in possible chemopreventive effects of curcumin. Curcumin inhibits tumor initiation by (TPA) in female CD-1 mice.


______________________________________ THE BIOLOGICAL ACTIVITIES OF CURCUMIN The biological activity Inhibition ______________________________________ 1. TPA-induced epidermal ornithine ++++ decarboxylase activity 2. TPA-induced edema of mouse ear ++++ 3. TPA-induced epidermal hyperplasia ++++ 4. TPA-induced epidermal DNA + synthesis 5. Arachidonic acid-induced edema of +++ mouse ear 6. Epidermal lipoxygenase ++++ activity 7. Epidermal cyclooxygenase activity ++++ 8. TPA-induced skin tumor promotion ++++ in previously initiated mice ______________________________________
Co-application of curcumin with TPA inhibits TPA-induced skin inflammation, increased epidermal ornithine decarboxylase activity. Huang et al. have also shown that 2; curcumin in the diet inhibits azoxymethane-induced foci in the colon of CF-1 mice and inhibits duodenal tumorigenesis in C57BL/6J mice previously initiated with N-ethyl-N'-nitro-N-nitrosguanidine.
Many of the anti-inflammatory and chemopreventive properties of curcumin may be related to the antioxidant properties of curcumin. In the present study, the antioxidant properties of curcumin, demethoxycurcumin, bisdemethoxycurcumin and several preparations containing varied proportions of curcumin, demethoxycurcumin, bisdemethoxycurcumin were measured by the Rancimat method.


______________________________________ ##STR1## R.sub.1 R.sub.2 ______________________________________ OCH.sub.3 OCH.sub.3 Curcumin H OCH.sub.3 Demethoxycurcumin H H Bisdemethoxycurcumin ______________________________________
The Rancimat method measures the conductivity changes caused by formation of small fatty acid molecules when the fats and oils are oxidized under elevated temperature and accelerated aeration. The induction times of lard with curcuminoids added were measured. The longer induction times suggest stronger antioxidant activity. All curcuminoids showed excellent antioxidant activity.
In chronic inflammation, cytokines induce the production of nitric oxide that is converted to DNA damaging and carcinogenic peroxynitrite and nitrite. The effect of curcumin on the generation of peroxynitrite radicals and nitrite was studied. Curcumin inhibited lipopolysaccharide (LPS) and interferon .gamma. (INF.gamma.) induced nitrite production by mouse peritoneal cells by more than 50% at 2.5-10 uM (FIG. 6).
The present invention is directed to a composition containing three curcuminoids, i.e., curcumin, demethoxy c

REFERENCES:
patent: 4263333 (1981-04-01), Maing et al.
patent: 4719111 (1988-01-01), Wilson
patent: 5108750 (1992-04-01), Liu
patent: 5120538 (1992-06-01), Oei
patent: 5266344 (1993-11-01), Mimura et al.
patent: 5401504 (1995-03-01), Das et al.
patent: 5401777 (1995-03-01), Ammon et al.
Biological Abstacts, vol. 100, Issue 12, pp. 2493-2497, "Effects of Curcumin . . . ", 1995.
Chem. Pharm. Bull. vol. 33, No. 4, pp. 1725-1728, "Natural Antioxidants . . . ", 1985.
Journal of Ethno-Pharmacology, vol. 38, pp. 113-119, "Mechanism of Antiinflammatory Actions of Curcumine and Boswellic Acids", 1993.

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