Bioproduction of astaxanthin using mutant carotenoid...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

Reexamination Certificate

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C435S069100, C435S183000, C435S252300, C435S252330, C435S858000, C435S320100, C536S023200

Reexamination Certificate

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07074604

ABSTRACT:
Protein engineered nucleic acid fragments encoding a CrtO ketolase and a CrtZ hydroxylase are provided with increased astaxanthin synthesis activity. Methods using the present nucleic acid fragments are also provided for increasing or altering astaxanthin production in suitable production hosts.

REFERENCES:
patent: 5684238 (1997-11-01), Ausich et al.
patent: 5811273 (1998-09-01), Misawa et al.
patent: 5916791 (1999-06-01), Hirschberg et al.
patent: 5965795 (1999-10-01), Hirschberg et al.
patent: 5972690 (1999-10-01), Misawa et al.
patent: 6218599 (2001-04-01), Hirschberg et al.
patent: 6291204 (2001-09-01), Pasamontes et al.
patent: 6677134 (2004-01-01), Pasamontes et al.
patent: 2002/0102631 (2002-08-01), Cunningham, Jr. et al.
patent: 2002/0147371 (2002-10-01), Hohmann et al.
patent: 2003/0100045 (2003-05-01), Cheng et al.
patent: 2004/0268439 (2004-12-01), Cheng et al.
patent: 1380415 (2002-11-01), None
patent: 0393690 (1990-10-01), None
patent: 2792335 (1999-04-01), None
patent: WO 99/07867 (1999-02-01), None
patent: WO 00/61764 (2000-10-01), None
patent: WO 02/079395 (2002-10-01), None
patent: WO 03/012056 (2003-02-01), None
patent: WO 03/016503 (2003-02-01), None
patent: WO 2004/029275 (2004-04-01), None
Nishida et al. Appl Environ Microbiol. Aug. 2005;71(8):4286-96.
U.S. Appl. No. 60/577,970, filed Jun. 8, 2004, Cheng et al.
B. Hundle et al., Functional assignment ofErwinia herbicolaEho10 carotenoid genes expressed inEscherichia coli, Mol. Gen. Genet., vol. 245:406-416, 1994.
Yuhong Zhou et al., Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase, Nucleic Acids Res., vol. 19(21):6052, 1991.
Yasukazu Nakamura et al., Complete Genome Structure ofGloeobacter violaceusPCC 7421, a Cyanobacterium that Lacks Thylakoids (Supplement), DNA Res., vol. 10 (Suppl):181-201, 2003.
Gregory Armstrong, Carotenoid Genetics and Biochemistry, Elsevier Press, vol. 2:321-352, 1999.
Sabine Steiger et al., Cloning of two carotenoid ketolase genes fromNostoc punctiformefor the heterologous production of canthaxanthin and astaxanthin, Biotechnology Letters, vol. 26:813-817, 2004.
Katsuhiro Sakano et al., Degradation of Endogenous Organic Acids Induced by Pi Uptake inCatharanthus roseusCells: Involvement of the Biochemical pH-Stat, Plant Cell Phys., vol. 39(6):615-619, 1998.
Norihiko Misawa et al., Elucidation of theErwinia uredovoraCarotenoid Biosynthetic Pathway by Functional Analysis of Gene Products Expressed inEscherichia coli, Journal of Bacteriology, vol. 172(12):6704-6712, Dec. 1990.
David W. Leung et al., A Method for Random Mutagenesis of a Defined DNA Segment Using a Modified Polymerase Chain Reaction, Technique-J. Methods in Cell and Mol. Biol., vol. 1(1):11-15, 1989.
Delphine Lagarde et al., The zeaxanthin biosynthesis enzyme beta-carotene hydroxylase is involved in myxoxanthophyll synthesis inSynechocystissp. PCC 6803, Febs Letters, vol. 454:247-251, 1999.
Hartmut Linden, Carotenoid hydroxylase fromHaematococcus pluvialis: cDNA sequence, regulation and functional complementation, Biochimica et Biophysica Acta, vol. 1446:203-212, 1999.
Georg Schnurr et al., Mapping of a carotenogenic gene cluster fromErwinia herbicolaand functional identification of six genes, FEMS Microbiology Letters, vol. 78:157-162, 1991.
Norihiko Misawa et al., Structure and Functional Analysis of a Marine Bacterial Carotenoid Biosynthesis Gene Cluster and Astaxanthin Biosynthetic Pathway Proposed at the Gene Level, J. of Bacteriology, vol. 177(22):6575-6584, 1995.
Luis Pasamontes et al., Isolation and characterization of the carotenoid biosynthesis genes ofFlavobacteriumsp. strain R1534, Gene, vol. 185:35-41, 1997.
Bhupinder S. Hundle et al., In vitro expression and activity of lycopene cyclase and beta-carotene hydroxylase fromErwinia herbicola, FEBS, vol. 315(3):329-334, 1993.
Li Tian et al., Characterization of a second carotenoid beta-hydroxylase gene fromArabidopsisand its relationship to the LUT1 locus, Plant Mol. Biol., vol. 47:379-388, 2001.
Laure Hannibal et al., Isolation and Characterization of Canthaxanthin Biosynthesis Genes from the Photosynthetic BacteriumBradyrhizobiumsp. Strain ORS278, J. of Bacteriology, vol. 182(13):3850-3853, 2000.
Zairen Sun et al., Differential expression of two isopentenyl pyrophosphate isomerases and enhanced carotenoid accumulation in a unicellular chlorophyte, PNAS, vol. 95:11482-11488, 1998.
Jurgen Breitenbach et al., Expression inEscherichia coliand properties of the carotene ketolase fromHaematococcus pluvialis, FEMS Microbiology Letters, vol. 140:241-246, 1996.
Delphine Lagarde et al., Increased Production of Zeaxanthin and Other Pigments by Application of Genetic Engineering Techniques toSynechocystissp. Strain PCC 6803, Applied and Environmental Microbiology, vol. 66(1):64-72, 2000.
Mark Harker et al., Biosynthesis of ketocarotenoids in transgenic cyanobacteria expressing the algal gene for beta-C-4-oxygenase, crtO, FEBS Letters, vol. 404:129-134, 1997.
Alexandre Melnikov et al., Random mutagenesis by recombinational capture of PCR products inBacillus subtilisandAcinetobacter calcoaceticus, Nucl. Acids Res., vol. 27(4):1056-1062, 1999.

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