Bioprocesses for preparing 7-ACA and 7-ADAC

Details Bioprocesses for preparing 7-ACA and 7-ADAC Bioprocesses for preparing 7-ACA and 7-ADAC

1999-06-28

2000-06-06

Achutamurthy, Ponnathapu

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound having a 1-thia-5-aza-bicyclo

435 49, 435 51, 435183, 435230, 435243, 43525411, 4352523, 4352545, 435935, 536 231, 536 232, 536 2374, C12P 3500, C12P 3502, C12P 3506, C12N 114, C12N 120

Patent

active

060717130

ABSTRACT:
A bioprocess for preparing adipoyl-7-ACA comprising the steps: (a) transforming cells of a strain of Penicillium chrysogenum which produces isopenicillin N with an expression vector containing DNA encoding an enzyme, having expandase activity capable of accepting adipoyl 6-APA as a substrate, an enzyme having hydroxylase activity capable of accepting adipoyl-7-ADCA as a substrate and an enzyme having acetyl transferase activity capable of accepting adipoyl 7-ADAC as a substrate; (b) culturing the transformed cells from step a) in a suitable culture medium containing an adipate feedstock, wherein said cells produce adipoyl 6-APA; and (c) culturing the transformed cells producing adipoyl 6-APA of step b) under conditions suitable for expression of said DNA encoding enzyme, thereby producing the end product adipoyl-7-ACA.

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