Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Reexamination Certificate
2007-01-02
2007-01-02
Navarro, Mark (Department: 1645)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
C530S387100, C530S388200, C530S413000, C530S866000, C530S867000
Reexamination Certificate
active
10414555
ABSTRACT:
A process for producing recombinant anti-botulinum toxin antibody comprising the steps of fermenting recombinantE. Colicells in broth, concentrating the cells by removing the broth, crushing the concentrated cells, separating a permeate derived from the crushed cells from cell debris, purifying a recombinant antibotulinum antibody (Fab) from said permeate, and separating said Fab from impurities by diafiltration.
REFERENCES:
patent: 5756670 (1998-05-01), Guss et al.
patent: 5932449 (1999-08-01), Emanuel et al.
patent: 6284488 (2001-09-01), Weir et al.
patent: 6566500 (2003-05-01), Vitetta et al.
patent: 6652857 (2003-11-01), Williams et al.
patent: 2002/0102613 (2002-08-01), Hoogenboom
patent: 2002/0155114 (2002-10-01), Marks et al.
patent: 2004/0175385 (2004-09-01), Marks et al.
patent: 2004/0219542 (2004-11-01), Houston et al.
patent: 2005/0042775 (2005-02-01), Pomato et al.
patent: 2006/0160184 (2006-07-01), Hoogenboom et al.
patent: 001054018 (2000-11-01), None
Crowe, J et al, Qiagen, Inc., Methods in Molecular Biology, vol. 31, 1994, paes 371-387, 6xHis-NiNTA chromatography as a superior technique in recombinant protein expression/purification.
Lindner, P et al, Methods: A comparison to Methods in Enzymology, vol. 4, pp. 41-56, 1992, Purification of Native proteins from the cytoplasm and periplasm ofEscherichia coliusing IMAC and Histidine tails: A comparison of Proteins and Protocols.
Schier, R et al, J. Mol. Biol., 1996, pp. 551-567, vol. 263.
Emanuel, Peter et al, Journal of Immunological Methods, vol. 193(1996) 189-197.
Bentley William E.
Olsen, II Gilbert G.
Biffoni Ulysses John
Navarro Mark
Portner Ginny Allen
The United States of America as represented by the Secretary of
LandOfFree
Bioprocess for the production of recombinant anti-botulinum... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Bioprocess for the production of recombinant anti-botulinum..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Bioprocess for the production of recombinant anti-botulinum... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3757876