Biopolymer sequences by MALDI granddaughter ion spectra

Chemistry: analytical and immunological testing – Nuclear magnetic resonance – electron spin resonance or other...

Reexamination Certificate

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Reexamination Certificate

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07396686

ABSTRACT:
The invention relates to the acquisition of spectra of biopolymers, especially of proteins, in tandem mass spectrometers with ionization using matrix-assisted laser desorption (MALDI) for the examination or determination of sequence patterns. The invention consists of a method of measuring granddaughter spectra of terminal fragment ions of the bipolymers in tandem mass spectrometers, wherein a so-called in-source fragmentation to generate a first generation of fragment or daughter ions of a biopolymer is coupled with a subsequent measurement of granddaughter ions, which have been obtained by a further fragmentation of a selected type of daughter ions. The method according to the invention enables the determination of the terminal sequences which are otherwise very difficult to measure.

REFERENCES:
patent: 5734161 (1998-03-01), Köster
patent: 6300627 (2001-10-01), Köster et al.
patent: 2 260632 (1992-10-01), None
patent: WO 98/03684 (1998-01-01), None
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Johnson, Richard S., et al., “Collision-Induced Fragmentation of (M+H)+Ions Of Peptides. Side Chain Specific Sequence Ions”, International Journal of Mass Spectrometry and Ion Processes, vol. 86, Elsevier Science Publishers B.V., 1988, pp. 137-154.
Reiber, Duane C., et al., “Unknown Peptide Sequencing Using Matrix-Assisted Laser Desorption/Ionization and In-Source Decay”, Analytical Chemistry, vol. 70, No. 6, American Chemical Society, Mar. 15, 1998, pp. 1214-1222.
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