Biopolymer production process

Textiles: fiber preparation

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195 31P, 195100, 195114, C12D 1304

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active

040060586

ABSTRACT:
An improved process for the production of a heteropolysaccharide by fermentation of a methanol containing culture medium with a microorganism of Methylomonas mucosa NRRL B-5696 wherein an alkali metal glycerophosphate is incorporated into the culture medium to provide a source of assimilable phosphate. An iron chelating agent selected from the group consisting of the alkali metal and the ferric/alkali metal salts of ethylenediaminetetraacetic acid may be added to the culture medium and N-tris-(hydroxymethyl)methyl glycine may be employed as a buffering agent. The heteropolysaccharide thus produced may be employed in the in vivo form to provide a viscous aqueous solution injected into a subterranean oil reservoir for mobility control purposes.

REFERENCES:
patent: 3878045 (1975-04-01), Tannahill et al.
patent: 3923782 (1975-12-01), Finn et al.
Martin; John Charles, "Batchwise Production of Biopolymer from Methanol Fermentation", Jan. 1974 (Master Thesis) Cornell University.
Levine et al., A Compilation of Culture Media, The Williams and Wilkins Co., Baltimore (1930), Qr. 71, L4, pp. 49, 463.
Frankel et al., Gradwohl's Clinical Laboratory Methods and Diagnosis, The C. V. Mosby Company, Saint Louis, 7th Ed., vol. 1, (1970), RB37G7, pp. 117, 118.
Foster, W. R., "A Low-Tension Waterflooding Process", Journal of Petroleum Technology, vol. 25 (Feb. 1973), pp. 205-210.
Laptewicz, Jr., Joseph Edward, "Biopolymer from a Methanol-Utilizing Bacterium", Aug. 1972 (Master Thesis), Cornell University.

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