Biopolymer and gene complex

Drug – bio-affecting and body treating compositions – Solid synthetic organic polymer as designated organic active... – Polymer from ethylenic monomers only

Reexamination Certificate

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C435S320100, C536S023100

Reexamination Certificate

active

08066978

ABSTRACT:
Disclosed herein is a biopolymer/gene complex for the aerosol delivery of a gene. The biopolymer/gene complex comprises a polyethyleneimine (PEI) having a substitution of glucose for at least a portion of the primary amino groups of the polyethyleneimine, and the content of the polyethyleneimine is 2-4 times the content of the gene. Also, disclosed is a lung cancer therapeutic agent comprising a biopolymer/gene complex including a polyethyleneimine (PEI) having a substitution of glucose for 30-40 mol % of the primary amino groups of the polyethyleneimine, and a gene encoding PTEN, in which the content of the polyethyleneimine is 2-4 times the content of the gene.

REFERENCES:
patent: WO 99/ 13915 (1999-03-01), None
Kichler A., 2004, J. Gene Med., vol. 6, pp. S3-S10.
Yin et al., 2008, Oncogene, vol. 27, pp. 5443-5453.
Kim et al., Nov. 1, 2004, Canc. Res., vol. 64, pp. 7971-7976.
Brognard et al., 2001, Canc. Res., vol. 61, pp. 3986-3997.
Fajac et al., 2003, J. Gene Med., vol. 5. pp. 38-48.
Li et al., 2003, World J. Gastroenterol., vol. 9(2), pp. 262-266.
Rudolph et al., ‘Jet nebulization of PEI/DNA polyplexes: physical stability and invitro gene delivery efficiency’, J. Gene Med. Jan.-Feb. 2002, vol. 4(1), Abstract.
Morimoto et al., ‘Molecular weight-dependent gene transfection activity of unmodified and galactosylated polyethyleneimine of hepatoma cells and mouse liver’, Molecul. Ther. Feb. 2003, vol. 7(2), Abstract.
Kunath et al., ‘Galactose-PEI-DNA complexes for targeted gene delivery: degree of substitution affects complex size and transfection efficiency’, J. Controlled Release, 2003, vol. 88, Abstract.

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