Biomolecule sample immobilization

Liquid purification or separation – Processes – Liquid/liquid solvent or colloidal extraction or diffusing...

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210650, 210651, 210691, 210781, 210782, 210806, 21032164, 21032167, 21032184, 210359, 2105021, 210514, 422 56, 422 60, 422 61, 422 72, 422101, 435 6, 436501, 436170, 436178, 530415, 935 77, B01D 6114, B01D 1508, B01D 6912

Patent

active

051240416

ABSTRACT:
A filter system for separating and binding biomolecules contained in a solution includes a first filter means for selectively passing components of the solution that are smaller than the biomolecules, thereby concentrating the biomolecules in the solution. Also included is a binding membrane means having an affinity for the biomolecules for binding the biomolecules to the binding membrane means, with the binding membrane means being located where the biomolecules are concentrated by the first filter means. In the preferred mode, the binding membrane means is chosen to selectively bind the biomolecule of interest. According to a preferred method of the invention, biomolecules from a sample volume are bound to a binding membrane by urging the sample volume against a biomolecule-binding support membrane that is in contact with a cut-off filter membrane such that the sample volume will pass through the biomolecule-binding support membrane before passing through the cut-off filter membrane. The concept is to concentrate the biomolecules of interest by means of the cut-off membrane in the vicinity of the binding membrane, thereby increasing the binding yield on the binding membrane. In the preferred mode, the sample volume is urged against the binding membrane by centrifugation.

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