Combinatorial chemistry technology: method – library – apparatus – Method of creating a library – Solid-phase synthesis or particular method of cleavage from...
Reexamination Certificate
2011-08-02
2011-08-02
Steele, Amber D. (Department: 1639)
Combinatorial chemistry technology: method, library, apparatus
Method of creating a library
Solid-phase synthesis or particular method of cleavage from...
C506S034000
Reexamination Certificate
active
07989396
ABSTRACT:
A highly specific and versatile surface chemistry for immobilization of amine-terminated probes is disclosed. A bi-layered polymer thin film serves as the platform for coupling the probes, which are preferably oligonucleotides. The process involves sequentially coating a substrate with polyamine and polyacid anhydride. Hydrolyzed polyacid anhydride groups may be converted to non-hydrolyzed groups at about 100° C. prior to probe attachment. The process of coating the substrate requires no harsh chemical pretreatment of substrates such as RCA or Piranha cleaning. In addition, simple thermal activation of the anhydride groups has a low requirement for storage, leading to a long shelf life of modified surfaces. The disclosed surface chemistry is especially compatible with microfabrication processes, and its effective application to magnetic biosensors is demonstrated.
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Pourmand Nader
Wang Shan X.
Yu Heng
Aston David J.
Peters Verny LLP
Steele Amber D.
The Board of Trustees of the Leland Stanford Junior University
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