Biomolecule and/or cellular arrays on metal surfaces

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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Details

C435S005000, C435S006120, C435S180000, C536S023100, C536S024100

Reexamination Certificate

active

07002004

ABSTRACT:
Disclosed is a process to construct multi-component biomolecule or cellular arrays suitable for use in SPR imaging studies of large molecule, cellular/molecular, and cell/cell interactions. Also disclosed are the resulting arrays. The success of the procedure hinges on the use of a reversible protecting group to modify reversibly ω-functionalized alkanethiols self-assembled on metal substrates. The arrays themselves include a metal substrate, a continuous layer of an identical ω-modified alkanthiol adhered to the metal substrate, and one or more discrete spots of biomolecules or cells directly bonded to the continuous layer of ω-modified alkenthiol. The areas of the continuous layer of ω-modified alkenthiol not covered by one of the discrete spots are covered by a background material resistant to non-specific protein binding.

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