Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an...
Patent
1997-04-07
1999-09-21
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an...
435181, 436524, 436532, 530811, 530816, C12N 1114, C12N 1106, G01N 33551, C07K 1714
Patent
active
059553359
ABSTRACT:
Biomaterial such as an enzyme, cell, antigen or antibody is immobilized by covalent bonding to a substrate having an Si.sub.3 N.sub.4 surface containing Si--NH.sub.2 groups that provide reactive NH.sub.2 groups. A heterobifunctional cross-linking agent having an NH.sub.2 -reactive group and a biomaterial-reactive group is reacted with the NH.sub.2 groups of the surface and then with the biomaterial or the heterobifunctional cross-linking agent is reacted with the biomaterial and then with the NH.sub.2 groups of the surface. The Si.sub.3 N.sub.4 surface containing Si--NH.sub.2 groups can be formed by precipitating on a substrate a 10-1000 nm thick Si.sub.3 N.sub.4 layer from a SiH.sub.4 /NH.sub.3 mixture, and providing reactive NH.sub.2 groups on the surface of the layer by subjecting the surface to hydrolyzing cleaning.
REFERENCES:
patent: 5234820 (1993-08-01), Wagner et al.
Jimbo, et al., Chemical Abstracts 109:89013, 1988.
Jimbo, et al., Journal of Molecular Electronics, vol. 4, 1988, pp. 111-118.
Kaupp Ulrich B.
Kordos Peter
Luth Hans
Schoning Michael J.
Thust Marion
Bach Klaus J.
Foschungszentrum Julich GmbH
Naff David M.
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