Stock material or miscellaneous articles – Surface property or characteristic of web – sheet or block – Surface modified glass
Patent
1997-10-03
1999-10-05
Nutter, Nathan M.
Stock material or miscellaneous articles
Surface property or characteristic of web, sheet or block
Surface modified glass
4284231, 623 1, 424422, 424423, 427 224, G01N 33543, C12N 500, C12N 1108, C12O 100
Patent
active
059621362
DESCRIPTION:
BRIEF SUMMARY
FIELD OF THE INVENTION
The present invention is related to a new biomaterial for the selective adhesion of cells or tissues.
The present invention is also related to the biosensor, the bioreactor, the diagnostic device, the tissue and/or the organ comprising said biomaterial.
The present invention is also related to the method for obtaining said biomaterial.
BACKGROUND OF THE INVENTION AND STATE OF THE ART
The polymeric structures are widely used as a solid support in the medical and biochemical fields.
For organs such as skin, mucosa, blood vessels, liver and spleen the functions of which cannot be replaced satisfactorily with artificial material alone, the trend to develop hybrid artificial organs of integrated cell type has been more popular in recent years.
In such case, an important issue resides in the selection and design of matrix materials on which cells are cultured. For example, it is well known that the adhesion, growth and proliferation of endothelial cells depend on the solid support offered to the cells.
Matrices are generally constituted of polymer materials and such polymer materials have conventionally been surface modified by a variety of processes.
Surface modification processes are schematically grouped in dry processes, representatively illustrated by plasma processes, arc processes, etc., and wet processes represented by coating, graft polymerization, etc.
The plasma processes include for example non reactive plasma processes based on the sputtering action of inactive ions such as argon, reactive plasma processes using active gas such as oxygen.
The surface matrix obtained by said plasma processes is homogeneously modified.
The coating is conducted by precoating an adhesive protein in solution from connective tissues such as collagen, fibronectin, etc. onto a matrix surface, thereby iiproving the adhesion of cells (endothelial cells, fibroblasts, etc.)
The documents J63196283 and J07108060 describe materials comprising a polymer (previously treated with U.V., electron beam or ion irradiation), coated with sugars, proteins or lipids. Said materials are used in culture of animal cells and for the diagnostic of cancer.
However, for the development of new biomaterials, it becomes important to produce heterogeneous surfaces with path such that preferential cell adhesion can occur. By such strategy it will be possible to guide the cell growth along preferential directions for specific applications such as nerve reconstruction.
In addition, for in vivo studies, it is better to obtain biomaterials whose chemical composition is closer to biological materials (biomaterials containing only carbon, hydrogen, oxygen and nitrogen).
AIMS OF THE INVENTION
The present invention aims to provide a new biomaterial for the selective adhesion of cells and/or tissues which do not present the drawbacks of the state of the art.
A main aim of the invention is to obtain a biomaterial allowing preferential cell and/or tissue adhesion in order to guide the cell and the tissue growth along preferential directions, particularly for specific applications such as nerve reconstruction.
Another aim of the invention is to obtain a biomaterial which is biocompatible.
Another aim of the invention is to obtain a biomaterial which may be used for the diagnostic of cancer.
A further aim of the invention is to provide an improved biosensor, bioreactor, diagnostic device, organ and/or tissue comprising the biomaterial according to the invention.
A last aim of the invention is to provide a method to obtain the biomaterial according to the invention which is reproducible and may be easily used on a industrial scale.
SUMMARY OF THE INVENTION
The present invention concerns a biomaterial for the selective adhesion of cell and/or tissue, which comprises a polymeric support having an heterogeneous surface conditioned with an extracellular matrix protein (or a portion thereof) and a surfactant.
A polymeric support having an heterogeneous surface means a polymeric support having surface modified areas and non-modified surfac
REFERENCES:
patent: 5055316 (1991-10-01), Hoffman et al.
patent: 5308704 (1994-05-01), Suzuki et al.
patent: 5607475 (1997-03-01), Cahalan et al.
Bertrand Patrick
Detrait Eric
Dewez Jean-Luc
Lhoest Jean-Benoit
Rouxhet Paul
Nutter Nathan M.
Universite Catholique de Louvain
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