Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1994-02-18
1996-02-13
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 4, 435 29, 435195, 435207, 436164, 436172, C12Q 134, C12N 938, G01N 2175, G01N 2176
Patent
active
054910692
ABSTRACT:
The present invention provides a biomarker system for the in vivo and in vitro assessment of cell senescence. In the method of the present invention, .beta.-galactosidase activity is utilized as a means by which cell senescence may be assessed either in in vitro cell cultures or in vivo.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis
patent: 4683202 (1987-07-01), Mullis
patent: 5302706 (1994-04-01), Smith
Jongkind et al. "Cell Softing & Microchemistry of Cultured Human Fibroblasts: Applications in Genetics & Aging Research" Cytometry 5(2) 182-187 1984.
Leroy et al. "The Effect of Aging on Acid B-D-Galactosidase In Human Liver Cell Lines" Biol. Cell 47(2) 235-238 1983.
Aizawa and Kurimoto, "Age-related cell surface changes in human diploid fibroblasts revealed by lectin-mediated red blood cell adsorption assay: a lectin survey," Mechanisms of Ageing and Development, 11:245-252 (1979).
Bosmann et al., "Loss of a critical neutral protease in ageing WI-38 cells," Nature, 261:499-501 (1976).
Busse et al., "Senescence-induced alteration in cell surface carbohydrates correlated using proton NMR spectroscopy and a lectin-based affinity-binding assay," Biochimica et Biophysica Acta, 984:183-187 (1989).
Goldstein, "Replicative senescence: The human fibroblast comes of age," Science, 249:1129-1133 (1990).
Hayflick, L., "The limited in vitro lifetime of human diploid cell strains," Experimental Cell Research, 37:614 (1965).
Hayflick and Moorhead, "The serial cultivation of human diploid cell strains," Experimental Cell Research, 25:585 (1961).
Klunder and Hulser, "B-galactosidase activity in transfected Ltk.sup.- cells is differentially regulated in monolayer and in spheroid cultures," Experimental Cell Research, 207:155-162 (1993).
McCormick and Campisi, "Cellular aging and senescence," Current Opinion in Cell Biology, 3:230-234 (1991).
Ning et al., "Tumor suppression by chromosome 11 is not due to cellular senescence," Experimental Cell Research, 192:220-226 (1991).
Ning et al., "Genetic analysis of indefinite division in human cells: Evidence for a cell senescence-related gene(s) on human chromosome 4," Proc. Natl. Acad. Sci., 88:5635-5639 (1991).
Ning and Pereira-Smith, "Molecular genetic approaches to the study of cellular senescence," Mutation Research, 256:303-310 (1991).
O'Brien et al., "Suppression of tumor growth by senescence in virally transformed human fibroblasts," Proc. Natl. Acad. Sci., 83:8659-8663 (1986).
Peacocke and Campisi, "Cellular senescence: A reflection of normal growth control, differentiation, or aging?" Journal of Cellular Biochemistry, 45:147-155 (1991).
Pereira-Smith et al., "Immortal phenotype of the HeLa variant D98 is recessive in hybrids formed with normal human fibroblasts," Journal of Cellular Physiology, 142:222-225 (1990).
Pereira-Smith and Smith, "Genetic analysis of indefinite division in human cells: Identification of four complementation groups," Proc. Natl. Acad. Sci., 85:6042-6046 (1988).
Pereira-Smith and Smith, "Evidence for the recessive nature of cellular immortality," Science, 221:964-966 (1983).
Porter, et al., "Novel monoclonal antibodies identify antigenic determinants unique to cellular senescence." Journal of Cellular Physiology, 142:425-433 (1990).
Rose, et al., "Expression of c-fos and AP-1 activity in senescent human fibroblasts is not sufficient for DNA synthesis," Journal of Cell Biology, 119:1045-1411 (1992).
Schulte-Hermann et al., "Promotion of spontaneous preneoplastic cells in rat liver as a possible explanation of tumor production by nonmutagenic compounds," Cancer Research 43:839-844 (1983).
Seshadri and Campisi, "Repression of c-fos Transcription and an altered genetic program in senescent human fibroblasts," Science, 247:205-209 (1990).
Smith and Pereira-Smith, "Genetic and molecular studies of cellular immortalization," Advances in Cancer Research, 54:63-77 (1990).
Smith and Pereira-Smith, "Altered gene expression during cellular aging," Genome 31:386-389 (1989).
Smith and Pereira-Smith, "Further studies on the genetic and biochemical basis of cellular senescence," Experimental Genetics, 24:372-381 (1989).
Spiering, et al., "Correlation between complementation group for immortality and DNA synthesis inhibitors," Experimental Cell Research, 195:541-545 (1991).
Stanulis-Praeger, "Cellular senescence revisited: A review," Mechanisms of Ageing and Development, 38:1-48 (1987).
Sugawara et al., "Induction of cellular senescence in immortalized cells by human chromosome 1," Science 24:707-710 (1990).
West, et al., "Replicative senescence of human skin fibroblasts correlates with a loss of regulations and overexpression of collagenase activity," Experimental Cell Research, 184:138-147 (1989).
Wright, et al., "Reversible cellular senescence: Implications for immortalization of normal human diploid fibroblasts," Molecular and Cellular Biology, 9:3088-3092 (1989).
Yang and Homsby, "Dissociation of senescence-associated changes in differentiated gene expression and replicative senescence i ncultured adrenocortical cells," J. Cell Sci., 94:757 (1989).
Campisi Judith
Dirmi Goberdhan P.
Peacocke Monica
Degen Nancy J.
The Regents of the University of California
Wityshyn Michael G.
LandOfFree
Biomarkers of cell senescence does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Biomarkers of cell senescence, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Biomarkers of cell senescence will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-239957