Chemistry: analytical and immunological testing – Biospecific ligand binding assay
Patent
1983-04-21
1986-08-05
Cockeram, Herbert S.
Chemistry: analytical and immunological testing
Biospecific ligand binding assay
435 8, 436 50, 436 56, 436172, 436544, 436546, 436800, G01N 2176, G01N 33532, G01N 33533, G01N 33536
Patent
active
046043640
ABSTRACT:
A tracer composition is described whereby a nonradioactive photon emitter is coupled to a ligand, antigen or antibody for use in various immunoassay methods of analysis. The photon emitters employed are bioluminescent proteins such as firefly or bacterial luciferase as well as other luciferases from various species. To synthesize the new tracer, these photon emitters are coupled to antigens or antibodies using coupling agents such as glutaraldehyde, CNBr, carbodiimide and others. The coupling method may also include intermediate materials such as polysaccharides, polypeptides, polyacrylamides and others coupled between the photon emitter and the antigen or antibody.
Methods are described for using this tracer in both competitive and noncompetitive immunoassays to detect proteins, hormones, drugs, viruses and the like. Detection is accomplished by activating the tracer in a bioluminescent reaction and measuring the emitted light by photometric instruments or by photographic film. This new tracer composition has the advantages of nonradioactivity, high sensitivity and less endogeneous background activity.
REFERENCES:
patent: 4104029 (1978-08-01), Maier
patent: 4230797 (1980-10-01), Boguslaski et al.
patent: 4246340 (1981-01-01), Lundin et al.
patent: 4412001 (1983-10-01), Baldwin et al.
Bojanowski Richard F.
Cockeram Herbert S.
LandOfFree
Bioluminescent tracer composition and method of use in immunoass does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Bioluminescent tracer composition and method of use in immunoass, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Bioluminescent tracer composition and method of use in immunoass will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-969033