Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving luciferase
Patent
1997-02-26
1999-04-06
Gitomer, Ralph
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving luciferase
435 15, 435 21, C12Q 166
Patent
active
058916598
ABSTRACT:
There is provided a bioluminescence reagent comprising at least pyruvate orthophosphate dikinase, phosphoenolpyruvic acid, pyrophosphoric acid, magnesium ion or another metallic ions, luciferin and luciferase, which reagent is such that the amount of luminescence is maintained in a high level and moreover stably without decaying for a long time in a bioluminescence reaction, and there is provided a method for quantitatively determining an adenosine phosphate ester or a substance taking part in the ATP conversion reaction in high sensitivity and high accuracy using an inexpensive and simple measuring apparatus.
REFERENCES:
patent: 4246340 (1981-01-01), Lundin et al.
patent: 4960696 (1990-10-01), Imahori et al.
Database WPI, AN 88-318 079, Derwent Publications Ltd., London; & JP-A-63 223 799 (Eisai Co., Ltd.) 1988.
Soviet Patents Abstracts, B section, week 8805, 1985, Aug. 30, Derwent Publications Ltd., London., & SU-A-1317 027 (Moscow Lomonosov Univ).
Eisaki Naoki
Imai Kazuhiro
Murakami Seiji
Nakajima Moto-o
Sakakibara Tatsuya
Gitomer Ralph
Kikkoman Corporation
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